Document Detail


Characterization of FRA7B, a human common fragile site mapped at the 7p chromosome terminal region.
MedLine Citation:
PMID:  20804921     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Common fragile sites (CFS) are specific regions of the mammalian chromosomes that are particularly prone to gaps and breaks. They are a cause of genome instability, and the location of many CFS correlates with breakpoints of aberrations recurrent in some cancers. The molecular characterization of some CFS has not clarified the causes of their fragility. In this work, by using fluorescence in situ hybridization analysis with BAC and PAC clones, we determined the DNA sequence of the CFS FRA7B. The FRA7B sequence was then analyzed to identify coding sequences and some structural features possibly involved in fragility. FRA7B spans about 12.2 megabases, and is therefore one of the largest CFS analyzed. It maps at the 7p21.3-22.3 chromosome bands, therefore at the interface of G- and R-band regions that are probably difficult to replicate. A 90-kilobase long sequence that presents very high flexibility values was identified at the very beginning of the more fragile CFS region. Three large genes (THSD7A, SDK1, and MAD1L1) and two miRNA genes (MIRN589 and MIRN339) map in the fragile region. The chromosome band 7p22 is a recurrent breakpoint in chromosome abnormalities in different types of neoplasm. FRA7B is the first characterized CFS located in a chromosome terminal region.
Authors:
Nazario Bosco; Franca Pelliccia; Angela Rocchi
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cancer genetics and cytogenetics     Volume:  202     ISSN:  1873-4456     ISO Abbreviation:  Cancer Genet. Cytogenet.     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2010-08-31     Completed Date:  2010-09-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7909240     Medline TA:  Cancer Genet Cytogenet     Country:  United States    
Other Details:
Languages:  eng     Pagination:  47-52     Citation Subset:  IM    
Copyright Information:
2010 Elsevier Inc. All rights reserved.
Affiliation:
Dipartimento di Genetica e Biologia Molecolare, Università La Sapienza, Rome, Italy.
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MeSH Terms
Descriptor/Qualifier:
Chromosome Banding
Chromosome Fragile Sites*
Chromosome Fragility
Chromosome Mapping / methods
Chromosomes, Human, Pair 7*
Genomic Instability / genetics
Humans
In Situ Hybridization, Fluorescence
Lymphocytes / cytology,  pathology,  physiology
MicroRNAs / genetics
Neoplasms / genetics
Chemical
Reg. No./Substance:
0/MicroRNAs

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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