| Characterization of enzymes in the oxidation of 1,2-propanediol to D: -(-)-lactic acid by Gluconobacter oxydans DSM 2003. | |
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MedLine Citation:
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PMID: 20300886 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Although Gluconobacter oxydans can convert 1,2-propanediol to D: -(-)-lactic acid, the enzyme(s) responsible for the conversion has remain unknown. In this study, the membrane-bound alcohol dehydrogenase (ADH) of Gluconobacter oxydans DSM 2003 was purified and confirmed to be essential for the process of D: -(-)-lactic acid production by gene knockout and complementation studies. A 25 percent decrease in D: -(-)-lactic acid production was found for the aldehyde dehydrogenase (ALDH) deficient strain of G. oxydans DSM 2003, indicating that this enzyme is involved in the reaction but not necessary. It is the first report that reveals the function of ADH and ALDH in the biooxidation of 1,2-propanediol to D: -(-)-lactic acid by G. oxydans DSM 2003. |
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Authors:
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Liujing Wei; Xuepeng Yang; Keliang Gao; Jinping Lin; Shengli Yang; Qiang Hua; Dongzhi Wei |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Molecular biotechnology Volume: 46 ISSN: 1559-0305 ISO Abbreviation: Mol. Biotechnol. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-04 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9423533 Medline TA: Mol Biotechnol Country: United States |
Other Details:
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Languages: eng Pagination: 26-33 Citation Subset: IM |
Affiliation:
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State Key Laboratory of Bioreactor Engineering, Newworld Institute of Biotechnology, East China University of Science and Technology, Shanghai, China. weiliujing@ecust.edu.cn |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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