| Characterization of the Effects of Exercise Training on Hematopoietic Stem Cell Quantity and Function. | |
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MedLine Citation:
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PMID: 23019311 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The effect of exercise training on hematopoietic stem cells (HSC) is largely unknown. The aim of the present investigation was to determine whether exercise training could expand the bone marrow HSC pool, and influence various aspects of HSC function. Mice were either exercise trained (EX; 1 hour/day, 3 days/week for 8 weeks) or remained sedentary (SED). Bone marrow (BM) from SED or EX mice was extracted from different HSC niches for cell cycle analysis, HSC (lineage(-), Sca-1(+), c-Kit(+); LSK) quantification, and differentiation along various hematopoietic lineages via flow cytometry. Serum was collected for evaluation of cytokines known to regulate HSC. To determine HSC function, BM from EX and SED mice was transplanted into primary and secondary recipients in a BM transplant (BMT) assay. EX increased HSC quantity in the vascular BM niche 20% versus SED (p<0.05), and increased the proportion of whole BM cells in G(2)/M phase of cell cycle (p<0.05). The number of spleen colonies was 48% greater (p<0.05) in recipients transplanted with BM from EX. Serum IL-6 levels were decreased 38% in EX, and differentiation along the lineage trended to increase (16%, p=0.053 and 16%, p=0.061, respectively). Short- or long-term engraftment and homing in primary recipients were not altered in EX. HSC self-renewal as analyzed by hematopoietic regeneration in secondary recipients was also unaffected by EX. Here we demonstrate that HSC quantity is increased in the BM niche associated with more activated, differentiated HSC, and that this expansion does not improve or impair HSC function. |
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Authors:
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Michael De Lisio; Gianni Parise |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-9-27 |
Journal Detail:
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Title: Journal of applied physiology (Bethesda, Md. : 1985) Volume: - ISSN: 1522-1601 ISO Abbreviation: J. Appl. Physiol. Publication Date: 2012 Sep |
Date Detail:
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Created Date: 2012-9-28 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8502536 Medline TA: J Appl Physiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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1McMaster University. |
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