Document Detail


Characterization of the effects of exercise training on hematopoietic stem cell quantity and function.
MedLine Citation:
PMID:  23019311     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of exercise training on hematopoietic stem cells (HSC) is largely unknown. The aim of the present investigation was to determine whether exercise training could expand the bone marrow HSC pool and influence various aspects of HSC function. Mice were either exercise trained (EX; 1 h/day, 3 days/wk, for 8 wk) or remained sedentary (SED). Bone marrow (BM) from SED or EX mice was extracted from different HSC niches for cell cycle analysis, HSC (lineage(-), Sca-1(+), c-Kit(+)) quantification, and differentiation along various hematopoietic lineages via flow cytometry. Serum was collected for evaluation of cytokines known to regulate HSC. To determine HSC function, BM from EX and SED mice was transplanted into primary and secondary recipients in a BM transplant assay. EX increased HSC quantity in the vascular BM niche 20% vs. SED (P < 0.05) and increased the proportion of whole BM cells in G(2)/M phase of cell cycle (P < 0.05). The number of spleen colonies was 48% greater (P < 0.05) in recipients transplanted with BM from EX. Serum IL-6 levels were decreased 38% in EX, and differentiation along the lineage trended to increase (16%, P = 0.053 and 16%, P = 0.061, respectively). Short- or long-term engraftment and homing in primary recipients were not altered in EX. HSC self-renewal as analyzed by hematopoietic regeneration in secondary recipients was also unaffected by EX. Here we demonstrate that HSC quantity is increased in the BM niche associated with more activated, differentiated HSC, and that this expansion does not improve or impair HSC function.
Authors:
Michael De Lisio; Gianni Parise
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-09-27
Journal Detail:
Title:  Journal of applied physiology (Bethesda, Md. : 1985)     Volume:  113     ISSN:  1522-1601     ISO Abbreviation:  J. Appl. Physiol.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-11-16     Completed Date:  2013-04-29     Revised Date:  2013-12-04    
Medline Journal Info:
Nlm Unique ID:  8502536     Medline TA:  J Appl Physiol (1985)     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1576-84     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, Ly / metabolism
Biological Markers / metabolism
Cell Differentiation
Cell Lineage
Cell Movement
Cell Proliferation
Cell Survival
Female
Flow Cytometry
G2 Phase Cell Cycle Checkpoints
Granulocyte Colony-Stimulating Factor / blood
Granulocyte-Macrophage Colony-Stimulating Factor / blood
Green Fluorescent Proteins / genetics,  metabolism
Hematopoietic Stem Cell Transplantation
Hematopoietic Stem Cells / immunology,  metabolism,  physiology*
Interleukin-3 / blood
Interleukin-6 / blood
Male
Membrane Proteins / metabolism
Mice
Mice, Inbred C57BL
Mice, Transgenic
Physical Exertion*
Proto-Oncogene Proteins c-kit / metabolism
Sedentary Lifestyle
Spleen / cytology
Stem Cell Niche*
Time Factors
Grant Support
ID/Acronym/Agency:
//Canadian Institutes of Health Research
Chemical
Reg. No./Substance:
0/Antigens, Ly; 0/Biological Markers; 0/Interleukin-3; 0/Interleukin-6; 0/Ly6a protein, mouse; 0/Membrane Proteins; 0/enhanced green fluorescent protein; 143011-72-7/Granulocyte Colony-Stimulating Factor; 147336-22-9/Green Fluorescent Proteins; 83869-56-1/Granulocyte-Macrophage Colony-Stimulating Factor; EC 2.7.10.1/Proto-Oncogene Proteins c-kit
Comments/Corrections

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