Document Detail

Characterization of the Drosophila caspase, DAMM.
MedLine Citation:
PMID:  11337486     Owner:  NLM     Status:  MEDLINE    
Caspases are main effectors of apoptosis in metazoans. Genome analysis indicates that there are seven caspases in Drosophila, six of which have been previously characterized. Here we describe the cloning and characterization of the last Drosophila caspase, DAMM. Similar to mammalian effector caspases, DAMM lacks a long prodomain. We show that the DAMM precursor, along with the caspases DRONC and DECAY, is partially processed in cells undergoing apoptosis. Recombinant DAMM produced in Escherichia coli shows significant catalytic activity on a pentapeptide caspase substrate. Low levels of damm mRNA are ubiquitously expressed in Drosophila embryos during early stages of development. Relatively high levels of damm mRNA are detected in larval salivary glands and midgut, and in adult egg chambers. Ectopic expression of DAMM in cultured cells induces apoptosis, and similarly, transgenic overexpression of DAMM, but not of a catalytically inactive DAMM mutant, in Drosophila results in a rough eye phenotype. We demonstrate that expression of the catalytically inactive DAMM mutant protein significantly suppresses the rough eye phenotype due to the overexpression of HID, suggesting that DAMM may be required in a hid-mediated cell death pathway.
N L Harvey; T Daish; K Mills; L Dorstyn; L M Quinn; S H Read; H Richardson; S Kumar
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2001-05-03
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  276     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2001 Jul 
Date Detail:
Created Date:  2001-07-02     Completed Date:  2001-08-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  25342-50     Citation Subset:  IM    
Hanson Centre for Cancer Research, Institute of Medical and Veterinary Science, Frome Road, Adelaide, SA 5000, Australia.
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MeSH Terms
Amino Acid Sequence
Caspases / chemistry*,  genetics,  metabolism
Cells, Cultured
Cloning, Molecular
Drosophila / enzymology*,  growth & development
Drosophila Proteins*
Escherichia coli
Eye / growth & development
Inhibitor of Apoptosis Proteins
Insect Proteins / metabolism
Molecular Sequence Data
RNA, Messenger / metabolism
Recombinant Proteins / metabolism
Reg. No./Substance:
0/DIAP2 protein, Drosophila; 0/Drosophila Proteins; 0/Inhibitor of Apoptosis Proteins; 0/Insect Proteins; 0/RNA, Messenger; 0/Recombinant Proteins; 0/thread protein, Drosophila; EC 3.4.22.-/Caspases; EC 3.4.22.-/DAMM protein, Drosophila; EC 3.4.22.-/Nc protein, Drosophila; EC 3.4.22.-/decay protein, Drosophila

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