Document Detail

Characterization of the DiFi rectal carcinoma cell line derived from a familial adenomatous polyposis patient.
MedLine Citation:
PMID:  8385096     Owner:  NLM     Status:  MEDLINE    
The DiFi human colorectal cancer cell line was recently established from a familial adenomatous polyposis patient with extracolonic features characteristic of the Gardner syndrome. These cells have now been propagated for 150 passages in standard culture media and vessels without feeder layers or collagen coatings. They retain features of colonic epithelial cells such as surface microvilli, secretory vesicles, and desmosomes. Cytosol of DiFi cells contains a high level (502 U/mg protein) of the mucin CA 19-9. In addition, DiFi cells produce carcinoembryonic antigen, and induce tumors in athymic mice. Cytoskeleton analysis of DiFi cells by fluorescence microscopy showed a pronounced disorganization of actin cable structure. The isozyme genetic signature of DiFi cells is unique (0.01 probability of finding the same genetic signature in a different cell line), differs from that of HeLa cells, and has expressional features seen in other colorectal cell lines. The DiFi cell karyotype is tetraploid, contains many marker chromosomes, and shows numerous episomal particles. Two copies of chromosome 18 were absent, and only a single normal chromosome 17 was found. This parallels detection of allelic losses from DiFi cell DNA at loci on chromosomes 17p and 18 using molecular (cDNA) probes. DiFi cells clearly express transcripts for the c-myc proto-oncogene, the c-myb proto-oncogene, and the p53 tumor suppressor gene. Transforming growth factor beta inhibits DiFi cell growth in soft agar and suppresses c-myc expression in these cells. The value of this cell line in the study of genetic alterations in colorectal cancer is discussed.
M Olive; S Untawale; R J Coffey; M J Siciliano; D M Wildrick; H Fritsche; S Pathak; L M Cherry; M Blick; P Lointier
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  In vitro cellular & developmental biology : journal of the Tissue Culture Association     Volume:  29A     ISSN:  0883-8364     ISO Abbreviation:  In Vitro Cell. Dev. Biol.     Publication Date:  1993 Mar 
Date Detail:
Created Date:  1993-05-04     Completed Date:  1993-05-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8506951     Medline TA:  In Vitro Cell Dev Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  239-48     Citation Subset:  IM    
University of Texas M. D. Anderson Cancer Center, Section of Gastrointestinal Oncology and Digestive Diseases, Houston 77030.
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MeSH Terms
Actins / ultrastructure
Adenocarcinoma, Mucinous / genetics*,  ultrastructure
Antigens, Tumor-Associated, Carbohydrate / analysis
Cell Cycle
Cell Line / chemistry,  drug effects
Cytoplasmic Granules / ultrastructure
Desmosomes / ultrastructure
Gene Expression Regulation, Neoplastic
Mice, Nude
Microscopy, Fluorescence
Middle Aged
Proto-Oncogene Proteins c-myc / genetics
Rectal Neoplasms / genetics*,  ultrastructure
Transforming Growth Factor beta / pharmacology
Reg. No./Substance:
0/Actins; 0/Antigens, Tumor-Associated, Carbohydrate; 0/Proto-Oncogene Proteins c-myc; 0/Transforming Growth Factor beta

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