| Characterization of Dental Epithelial Stem Cells from the Mouse Incisor with 2D and 3D Platforms. | |
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MedLine Citation:
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PMID: 22742471 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Dental epithelial stem cells drive continuous growth in the adult mouse incisor. To date, no robust system for the primary culture of these cells has been reported, and little is known about the basic molecular architecture of these cells or the minimal extracellular scaffolding necessary to maintain the epithelial stem cell population in an undifferentiated state. We report a method to isolate dental epithelial stem cells from the cervical loop of the mouse mandibular incisor. Cells were viable in a two dimensional (2D) culture system and did not demonstrate preferential proliferation atop various substrates. Characterization of these cells indicated that E-cadherin, integrin alpha-6, and integrin beta-4 mark the dental epithelial stem cells both in vivo and in vitro. We also grew these cells in a three dimensional (3D) microenvironment and obtained spheres with epithelial morphology and expression patterns. Insights into the mechanisms of stem cell maintenance in vitro will help to lay the groundwork for the successful generation of bioengineered teeth from adult dental epithelial stem cells. |
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Authors:
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Miquella G Chavez; Wenli Yu; Brian Biehs; Hidemitsu Harada; Malcolm L Snead; Ophir D Klein |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-6-29 |
Journal Detail:
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Title: Tissue engineering. Part C, Methods Volume: - ISSN: 1937-3392 ISO Abbreviation: - Publication Date: 2012 Jun |
Date Detail:
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Created Date: 2012-6-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101466663 Medline TA: Tissue Eng Part C Methods Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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University of California, San Francisco, Department of Bioengineering and Therapeutic Sciences, 1700 4th Street, Mail Code 2520, San Francisco, California, United States, 94158-2230, 415-514-9695, 415-476-2414; miquella.chavez@ucsf.edu. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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