Document Detail


Characterization of dental epithelial stem cells from the mouse incisor with two-dimensional and three-dimensional platforms.
MedLine Citation:
PMID:  22742471     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Dental epithelial stem cells (DESCs) drive continuous growth in the adult mouse incisors. To date, a robust system for the primary culture of these cells has not been reported, and little is known about the basic molecular architecture of these cells or the minimal extracellular scaffolding that is necessary to maintain the epithelial stem cell population in an undifferentiated state. We report a method of isolating DESCs from the cervical loop of the mouse mandibular incisor. Cells were viable in a two-dimensional culture system and did not demonstrate preferential proliferation when grown on top of various substrates. Characterization of these cells indicated that E-cadherin, integrin alpha-6, and integrin beta-4 mark the DESCs both in vivo and in vitro. We also grew these cells in a three-dimensional microenvironment and obtained spheres with an epithelial morphology and expression patterns. Insights into the mechanisms of stem cell maintenance in vitro will help lay the groundwork for the successful generation of bioengineered teeth from adult DESCs.
Authors:
Miquella G Chavez; Wenli Yu; Brian Biehs; Hidemitsu Harada; Malcolm L Snead; Janice S Lee; Tejal A Desai; Ophir D Klein
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-08-16
Journal Detail:
Title:  Tissue engineering. Part C, Methods     Volume:  19     ISSN:  1937-3392     ISO Abbreviation:  Tissue Eng Part C Methods     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-12-11     Completed Date:  2013-09-16     Revised Date:  2014-03-19    
Medline Journal Info:
Nlm Unique ID:  101466663     Medline TA:  Tissue Eng Part C Methods     Country:  United States    
Other Details:
Languages:  eng     Pagination:  15-24     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Adhesion
Cell Culture Techniques / methods*
Cell Proliferation
Cell-Matrix Junctions / metabolism
Cells, Cultured
Epithelial Cells / cytology*
Incisor / cytology*
Intercellular Junctions / metabolism
Mice
Mice, Inbred C57BL
Spheroids, Cellular / cytology,  metabolism
Stem Cells / cytology*
Grant Support
ID/Acronym/Agency:
1-DP2-OD007191/OD/NIH HHS; DE06988/DE/NIDCR NIH HHS; K12 GM081266/GM/NIGMS NIH HHS; K12GM081266/GM/NIGMS NIH HHS; P30 DK063720/DK/NIDDK NIH HHS; R01 DE021420/DE/NIDCR NIH HHS; R01-DE021420/DE/NIDCR NIH HHS
Comments/Corrections

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