| Characterization of DNA hybridization on partially aminated diamond by aromatic compounds. | |
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MedLine Citation:
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PMID: 17154611 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Here, we report a novel method of micropatterning oligonucleotides via aromatic groups as linkers on partially amino-terminated diamond and the inherence on subsequent hybridization. The covalent immobilization of probe oligonucleotides and characterization of immobilized probe oligonucleotides with carboxylic compounds were investigated by X-ray photoelectron spectroscopy (XPS). To confirm the effects of linker flexibility in a low amino group on diamond for probe oligonucleotides, three kinds of dicarboxylic compound--adipic acid, terephthalic acid, and trimesic acid--were used for immobilization of probe oligonucleotides, like linkers; and these oligonucleotides were hybridized with target oligonucleotides labeled with Cy 5 on the micropatterned diamond surface. The hybridization intensities determined by epifluorescence microscopy were compared and analyzed. |
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Authors:
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Jung-Hoon Yang; Kwang-Soup Song; Guo-Jun Zhang; Munenori Degawa; Yoshinori Sasaki; Iwao Ohdomari; Hiroshi Kawarada |
Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Langmuir : the ACS journal of surfaces and colloids Volume: 22 ISSN: 0743-7463 ISO Abbreviation: Langmuir Publication Date: 2006 Dec |
Date Detail:
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Created Date: 2006-12-12 Completed Date: 2007-01-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9882736 Medline TA: Langmuir Country: United States |
Other Details:
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Languages: eng Pagination: 11245-50 Citation Subset: IM |
Affiliation:
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School of Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Carbocyanines
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chemistry* Cross-Linking Reagents / chemistry* Diamond / chemistry* Nucleic Acid Hybridization / methods Oligonucleotide Array Sequence Analysis* / methods Oligonucleotides / chemistry* Spectrometry, Fluorescence / methods Spectrometry, X-Ray Emission / methods |
| Chemical | |
Reg. No./Substance:
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0/Carbocyanines; 0/Cross-Linking Reagents; 0/Oligonucleotides; 0/cyanine dye 5; 7782-40-3/Diamond |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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