| Characterization of D-ribose biosynthesis in Bacillus subtilis JY200 deficient in transketolase gene. | |
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MedLine Citation:
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PMID: 16143417 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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D-Ribose is a functional five-carbon sugar, which has been used for the commercial production of riboflavin. Mechanisms of d-ribose biosynthesis from xylose were investigated in the genetically engineered Bacillus subtilis JY200 with a deficiency in transketolase. A transketolase gene (tkt) disruption cassette in plasmid pUNKC was introduced into the chromosomal tkt gene in the wild type B. subtilis 168. Analysis of culture broth by thin layer chromatography confirmed that the disruption of tkt allowed B. subtilis JY200 to produce d-ribose. In a batch culture of B. subtilis JY200, a loss of cell viability was observed after glucose depletion. Fed-batch cultivation by feeding 400 gl(-1) glucose solution as a co-substrate was carried out to supply energy to xylose metabolism and to maintain cell viability throughout cultivation. Fed-batch cultivation of B. subtilis JY200 in a complex medium containing 11 gl(-1) xylose and 5 gl(-1) glucose initially gave the best result of 10.1 gl(-1)D-ribose concentration, 0.24 gg(-1)D-ribose yield and 0.29 gl(-1)h(-1) productivity, corresponding to 40-, 5- and 12-fold increases compared with those in the batch culture. A kinetic study of D-ribose production in fed-batch cultivations of B. subtilis JY200 suggested that xylose uptake might be critical to maximize D-ribose biosynthesis from xylose. |
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Authors:
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Yong-Cheol Park; Jin-Ho Choi; George N Bennett; Jin-Ho Seo |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-09-06 |
Journal Detail:
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Title: Journal of biotechnology Volume: 121 ISSN: 0168-1656 ISO Abbreviation: J. Biotechnol. Publication Date: 2006 Feb |
Date Detail:
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Created Date: 2006-02-13 Completed Date: 2006-05-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8411927 Medline TA: J Biotechnol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 508-16 Citation Subset: IM |
Affiliation:
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Interdisciplinary Program for Biochemical Engineering and Biotechnology, Seoul National University, Seoul 151-742, Republic of Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacillus subtilis
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genetics,
growth & development* Bacterial Proteins / genetics Gene Deletion Genes, Bacterial / genetics Riboflavin / chemical synthesis, chemistry Ribose / analysis, biosynthesis*, chemistry Transketolase / genetics Xylose / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Xylose; 50-69-1/Ribose; 83-88-5/Riboflavin; EC 2.2.1.1/Transketolase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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