| Characterization of D: -lactate dehydrogenase from Pediococcus acidilactici that converts phenylpyruvic acid into phenyllactic acid. | |
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MedLine Citation:
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PMID: 22261863 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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The gene coding for D: -lactate dehydrogenase (D: -LDH) from Pediococcus acidilactici DSM 20284 was cloned and expressed in E. coli. The recombinant enzyme was purified by nickel-affinity chromatography. It converted phenylpyruvic acid (PPA) to 3-phenyllactic acid maximally at 30°C and pH 5.5 with a specific activity of 140 and 422 U/mg for PPA and pyruvate, respectively. The K (m), turnover number (k (cat)), and catalytic efficiency (k (cat)/K (m)) for PPA were 2.9 mM, 305 s(-1), and 105 mM(-1) s(-1), respectively. |
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Authors:
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Wanmeng Mu; Shuhuai Yu; Bo Jiang; Xingfeng Li |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-1-20 |
Journal Detail:
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Title: Biotechnology letters Volume: - ISSN: 1573-6776 ISO Abbreviation: - Publication Date: 2012 Jan |
Date Detail:
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Created Date: 2012-1-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8008051 Medline TA: Biotechnol Lett Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, Jiangsu, China, wmmu@jiangnan.edu.cn. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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