| Characterization of the Cell-Nanopillar Interface by Transmission Electron Microscopy. | |
| | |
MedLine Citation:
|
PMID: 23030066 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Vertically aligned nanopillars can serve as excellent electrical, optical and mechanical platforms for biological studies. However, revealing the nature of the interface between the cell and the nanopillar is very challenging. In particular, a matter of debate is whether the cell membrane remains intact around the nanopillar. Here we present a detailed characterization of the cell-nanopillar interface by transmission electron microscopy. We examined cortical neurons growing on nanopillars with diameter 50-500 nm and heights 0.5-2 µm. We found that on nanopillars less than 300 nm in diameter, the cell membrane wraps around the entirety of the nanopillar without the nanopillar penetrating into the interior of the cell. On the other hand, the cell sits on top of arrays of larger, closely spaced nanopillars. We also observed that the membrane-surface gap of both cell bodies and neurites is smaller for nanopillars than for a flat substrate. These results support a tight interaction between the cell membrane and the nanopillars and previous findings of excellent sealing in electrophysiology recordings using nanopillar electrodes. |
| | |
Authors:
|
Lindsey Hanson; Ziliang Carter Lin; Chong Xie; Yi Cui; Bianxiao Cui |
Related Documents
:
|
23473796 - Microwave processing of gustatory tissues for immunohistochemistry. 23638186 - Small gtpase rab40c associates with lipid droplets and modulates the biogenesis of lipi... 23286696 - Repressive effects of a capacitive-resistive electric transfer (cret) hyperthermic appa... 23157596 - Phb granules are attached to the nucleoid via pham in ralstonia eutropha. 23473796 - Microwave processing of gustatory tissues for immunohistochemistry. 9573196 - The prosequence of pro-sigmak promotes membrane association and inhibits rna polymerase... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-10-3 |
Journal Detail:
|
Title: Nano letters Volume: - ISSN: 1530-6992 ISO Abbreviation: Nano Lett. Publication Date: 2012 Oct |
Date Detail:
|
Created Date: 2012-10-3 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101088070 Medline TA: Nano Lett Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Taking the F out of FLP. Simple Lewis acid-base pairs for mild reductions with neutral boranes via b...
Next Document: The combined effect of persistent organic pollutants in the serum POP mixture in Greenlandic Inuit: ...