Document Detail


Characterization of CFTR expression in a human pulmonary mucoepidermoid carcinoma cell line, NCI-H292 cells.
MedLine Citation:
PMID:  10437775     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The NCI-H292 cell, a human pulmonary mucoepidermoid carcinoma cell line, is commonly used for studying bacterial and viral infections of airway epithelial cells. Dysfunction of the cystic fibrosis transmembrane conductance regulator (CFTR) is the main cause of fetal lung infection in cystic fibrosis patients. In this study, we examined CFTR expression in NCI-H292 cells to determine whether NCI-H292 cells possess sufficient, normally functioning CFTR. The results of RT-PCR and Northern blotting analysis indicated that the CFTR gene expression level was much lower in NCI-H292 cells than in T84 cells. However, Western blotting analysis showed that protein expression in NCI-H292 cells was comparable to that in T84 cells. Furthermore, whole-cell and cell-attached patch clamp electrophysiological techniques indicated that the Cl- current induced by intracellular cAMP elevation in NCI-H292 cells was comparable to that in T84 cells. These findings suggest that NCI-H292 cells with a low level of CFTR gene expression possess enough functional CFTR to show a physiological response.
Authors:
S Nagayama; H Kai; T Okiyoneda; S Horikawa; T Miyata
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  455     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1999 Jul 
Date Detail:
Created Date:  1999-08-16     Completed Date:  1999-08-16     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  215-8     Citation Subset:  IM    
Affiliation:
Department of Pharmacological Sciences, Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Blotting, Northern
Carcinoma, Mucoepidermoid / genetics,  metabolism
Cyclic AMP / metabolism
Cystic Fibrosis Transmembrane Conductance Regulator / drug effects,  genetics*,  metabolism*
DNA Primers / genetics
Forskolin / pharmacology
Gene Expression
Humans
Lung Neoplasms / genetics,  metabolism
Patch-Clamp Techniques
RNA, Messenger / genetics,  metabolism
Respiratory Tract Infections / etiology,  genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/CFTR protein, human; 0/DNA Primers; 0/RNA, Messenger; 126880-72-6/Cystic Fibrosis Transmembrane Conductance Regulator; 60-92-4/Cyclic AMP; 66428-89-5/Forskolin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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