Document Detail

Characterization of CD133+ parenchymal cells in the liver: histology and culture.
MedLine Citation:
PMID:  19842219     Owner:  NLM     Status:  MEDLINE    
AIM: To reveal the characteristics of CD133(+) cells in the liver.
METHODS: This study examined the histological characteristics of CD133(+) cells in non-neoplastic and neoplastic liver tissues by immunostaining, and also analyzed the biological characteristics of CD133(+) cells derived from human hepatocellular carcinoma (HCC) or cholangiocarcinoma cell lines.
RESULTS: Immunostaining revealed constant expression of CD133 in non-neoplastic and neoplastic biliary epithelium, and these cells had the immunophenotype CD133(+)/CK19(+)/HepPar-1(-). A small number of CD133(+)/CK19(-)/HepPar-1(+) cells were also identified in HCC and combined hepatocellular and cholangiocarcinoma. In addition, small ductal structures, resembling the canal of Hering, partly surrounded by hepatocytes were positive for CD133. CD133 expression was observed in three HCC (HuH7, PLC5 and HepG2) and two cholangiocarcinoma cell lines (HuCCT1 and CCKS1). Fluorescence-activated cell sorting (FACS) revealed that CD133(+) and CD133(-) cells derived from HuH7 and HuCCT1 cells similarly produced CD133(+) and CD133(-) cells during subculture. To examine the relationship between CD133(+) cells and the side population (SP) phenotype, FACS was performed using Hoechst 33342 and a monoclonal antibody against CD133. The ratios of CD133(+)/CD133(-) cells were almost identical in the SP and non-SP in HuH7. In addition, four different cellular populations (SP/CD133(+), SP/CD133(-), non-SP/CD133(+), and non-SP/CD133(-)) could similarly produce CD133(+) and CD133(-) cells during subculture.
CONCLUSION: This study revealed that CD133 could be a biliary and progenitor cell marker in vivo. However, CD133 alone is not sufficient to detect tumor-initiating cells in cell lines.
Seiichi Yoshikawa; Yoh Zen; Takahiko Fujii; Yasunori Sato; Tetsuo Ohta; Yutaka Aoyagi; Yasuni Nakanuma
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  15     ISSN:  2219-2840     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-10-20     Completed Date:  2010-01-07     Revised Date:  2014-05-20    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  4896-906     Citation Subset:  IM    
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MeSH Terms
Antigens, CD / genetics,  metabolism*
Bile Duct Neoplasms / genetics,  immunology*,  pathology
Bile Ducts, Intrahepatic / immunology*,  pathology
Carcinoma, Hepatocellular / genetics,  immunology*,  pathology
Cell Line, Tumor
Cell Proliferation
Cell Separation
Cholangiocarcinoma / genetics,  immunology*,  pathology
Epithelial Cells / immunology*,  pathology
Flow Cytometry
Gene Expression Regulation, Neoplastic
Glycoproteins / genetics,  metabolism*
Keratin-19 / metabolism
Liver / immunology*,  pathology
Liver Neoplasms / genetics,  immunology*,  pathology
Middle Aged
Peptides / genetics,  metabolism*
Polymerase Chain Reaction
RNA, Messenger / metabolism
Time Factors
alpha-Fetoproteins / metabolism
Reg. No./Substance:
0/AC133 antigen; 0/AFP protein, human; 0/Antigens, CD; 0/Glycoproteins; 0/Keratin-19; 0/Peptides; 0/RNA, Messenger; 0/alpha-Fetoproteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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