Document Detail


Characterization of CD133+ parenchymal cells in the liver: histology and culture.
MedLine Citation:
PMID:  19842219     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To reveal the characteristics of CD133(+) cells in the liver. METHODS: This study examined the histological characteristics of CD133(+) cells in non-neoplastic and neoplastic liver tissues by immunostaining, and also analyzed the biological characteristics of CD133(+) cells derived from human hepatocellular carcinoma (HCC) or cholangiocarcinoma cell lines. RESULTS: Immunostaining revealed constant expression of CD133 in non-neoplastic and neoplastic biliary epithelium, and these cells had the immunophenotype CD133(+)/CK19(+)/HepPar-1(-). A small number of CD133(+)/CK19(-)/HepPar-1(+) cells were also identified in HCC and combined hepatocellular and cholangiocarcinoma. In addition, small ductal structures, resembling the canal of Hering, partly surrounded by hepatocytes were positive for CD133. CD133 expression was observed in three HCC (HuH7, PLC5 and HepG2) and two cholangiocarcinoma cell lines (HuCCT1 and CCKS1). Fluorescence-activated cell sorting (FACS) revealed that CD133(+) and CD133(-) cells derived from HuH7 and HuCCT1 cells similarly produced CD133(+) and CD133(-) cells during subculture. To examine the relationship between CD133(+) cells and the side population (SP) phenotype, FACS was performed using Hoechst 33342 and a monoclonal antibody against CD133. The ratios of CD133(+)/CD133(-) cells were almost identical in the SP and non-SP in HuH7. In addition, four different cellular populations (SP/CD133(+), SP/CD133(-), non-SP/CD133(+), and non-SP/CD133(-)) could similarly produce CD133(+) and CD133(-) cells during subculture. CONCLUSION: This study revealed that CD133 could be a biliary and progenitor cell marker in vivo. However, CD133 alone is not sufficient to detect tumor-initiating cells in cell lines.
Authors:
Seiichi Yoshikawa; Yoh Zen; Takahiko Fujii; Yasunori Sato; Tetsuo Ohta; Yutaka Aoyagi; Yasuni Nakanuma
Related Documents :
22592999 - Piperlongumine induces rapid depletion of the androgen receptor in human prostate cance...
15466389 - Activated hepatic stellate cells express keratinocyte growth factor in chronic liver di...
19932139 - Hepatocyte-like cells derived from human embryonic stem cells specifically via definiti...
12490299 - Embryonic liver cells and permanent lines as models for hepatocyte and bile duct cell d...
19867769 - An experimental study of the histogenesis of the miliary tubercle in vitally stained ra...
7834639 - High rates of thioredoxin secretion correlate with growth arrest in hepatoma cells.
20732499 - Human thymus mesenchymal stromal cells augment force production in self-organized cardi...
7208099 - Schistosoma mansoni: the sustentacular cells of the testes.
22592999 - Piperlongumine induces rapid depletion of the androgen receptor in human prostate cance...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  15     ISSN:  1007-9327     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-10-20     Completed Date:  2010-01-07     Revised Date:  2010-09-27    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  4896-906     Citation Subset:  IM    
Affiliation:
Department of Human Pathology, Kanazawa University Graduate School of Medicine, 13-1 Takaramachi, Kanazawa 920-8640, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Antigens, CD / genetics,  metabolism*
Bile Duct Neoplasms / genetics,  immunology*,  pathology
Bile Ducts, Intrahepatic / immunology*,  pathology
Carcinoma, Hepatocellular / genetics,  immunology*,  pathology
Cell Line, Tumor
Cell Proliferation
Cell Separation
Cholangiocarcinoma / genetics,  immunology*,  pathology
Epithelial Cells / immunology*,  pathology
Female
Flow Cytometry
Gene Expression Regulation, Neoplastic
Glycoproteins / genetics,  metabolism*
Humans
Immunohistochemistry
Keratin-19 / metabolism
Liver / immunology*,  pathology
Liver Neoplasms / genetics,  immunology*,  pathology
Male
Middle Aged
Peptides / genetics,  metabolism*
Phenotype
Polymerase Chain Reaction
RNA, Messenger / metabolism
Time Factors
alpha-Fetoproteins / metabolism
Chemical
Reg. No./Substance:
0/AC133 antigen; 0/AFP protein, human; 0/Antigens, CD; 0/Glycoproteins; 0/Keratin-19; 0/Peptides; 0/RNA, Messenger; 0/alpha-Fetoproteins
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Systematic review of Chinese herbal medicine for functional constipation.
Next Document:  Short-term Celecoxib intervention is a safe and effective chemopreventive for gastric carcinogenesis...