|Characterization of CD133+ parenchymal cells in the liver: histology and culture.|
|PMID: 19842219 Owner: NLM Status: MEDLINE|
|AIM: To reveal the characteristics of CD133(+) cells in the liver.
METHODS: This study examined the histological characteristics of CD133(+) cells in non-neoplastic and neoplastic liver tissues by immunostaining, and also analyzed the biological characteristics of CD133(+) cells derived from human hepatocellular carcinoma (HCC) or cholangiocarcinoma cell lines.
RESULTS: Immunostaining revealed constant expression of CD133 in non-neoplastic and neoplastic biliary epithelium, and these cells had the immunophenotype CD133(+)/CK19(+)/HepPar-1(-). A small number of CD133(+)/CK19(-)/HepPar-1(+) cells were also identified in HCC and combined hepatocellular and cholangiocarcinoma. In addition, small ductal structures, resembling the canal of Hering, partly surrounded by hepatocytes were positive for CD133. CD133 expression was observed in three HCC (HuH7, PLC5 and HepG2) and two cholangiocarcinoma cell lines (HuCCT1 and CCKS1). Fluorescence-activated cell sorting (FACS) revealed that CD133(+) and CD133(-) cells derived from HuH7 and HuCCT1 cells similarly produced CD133(+) and CD133(-) cells during subculture. To examine the relationship between CD133(+) cells and the side population (SP) phenotype, FACS was performed using Hoechst 33342 and a monoclonal antibody against CD133. The ratios of CD133(+)/CD133(-) cells were almost identical in the SP and non-SP in HuH7. In addition, four different cellular populations (SP/CD133(+), SP/CD133(-), non-SP/CD133(+), and non-SP/CD133(-)) could similarly produce CD133(+) and CD133(-) cells during subculture.
CONCLUSION: This study revealed that CD133 could be a biliary and progenitor cell marker in vivo. However, CD133 alone is not sufficient to detect tumor-initiating cells in cell lines.
|Seiichi Yoshikawa; Yoh Zen; Takahiko Fujii; Yasunori Sato; Tetsuo Ohta; Yutaka Aoyagi; Yasuni Nakanuma|
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|Type: Journal Article|
|Title: World journal of gastroenterology : WJG Volume: 15 ISSN: 2219-2840 ISO Abbreviation: World J. Gastroenterol. Publication Date: 2009 Oct|
|Created Date: 2009-10-20 Completed Date: 2010-01-07 Revised Date: 2014-05-20|
Medline Journal Info:
|Nlm Unique ID: 100883448 Medline TA: World J Gastroenterol Country: China|
|Languages: eng Pagination: 4896-906 Citation Subset: IM|
|APA/MLA Format Download EndNote Download BibTex|
Bile Duct Neoplasms / genetics, immunology*, pathology
Bile Ducts, Intrahepatic / immunology*, pathology
Carcinoma, Hepatocellular / genetics, immunology*, pathology
Cell Line, Tumor
Cholangiocarcinoma / genetics, immunology*, pathology
Epithelial Cells / immunology*, pathology
Gene Expression Regulation, Neoplastic
Glycoproteins / genetics, metabolism*
Keratin-19 / metabolism
Liver / immunology*, pathology
Liver Neoplasms / genetics, immunology*, pathology
Peptides / genetics, metabolism*
Polymerase Chain Reaction
RNA, Messenger / metabolism
alpha-Fetoproteins / metabolism
|0/AC133 antigen; 0/AFP protein, human; 0/Antigens, CD; 0/Glycoproteins; 0/Keratin-19; 0/Peptides; 0/RNA, Messenger; 0/alpha-Fetoproteins|
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