Document Detail

Characterization of both polyunsaturated fatty acid biosynthetic pathways in Schizochytrium sp.
MedLine Citation:
PMID:  19495823     Owner:  NLM     Status:  MEDLINE    
Schizochytrium produces long chain polyunsaturated fatty acids (PUFAs) via a PUFA synthase. Targeted mutagenesis of one gene of this synthase was conducted to confirm PUFA synthase function and determine its metabolic necessity. The resulting mutants were auxotrophic and required supplementation with PUFAs. In vivo labeling experiments with radioactive fatty acids demonstrated the presence of several elongase and desaturase activities associated with the standard pathway of PUFA synthesis. However, this system was missing a critical Delta12 desaturase activity and was therefore not capable of synthesizing PUFAs from the 16- or 18-carbon saturated fatty acid products of the fatty acid synthase. Because Schizochytrium uses a PUFA synthase system for the production of PUFAs, the existence of a partial desaturase-elongase system (if not a simple vestige) is suggested to be either a scavenging mechanism for intermediate fatty acids prematurely released by the PUFA synthase or for PUFAs found in the organism's native environment.
J Casey Lippmeier; Kristine S Crawford; Carole B Owen; Angie A Rivas; James G Metz; Kirk E Apt
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Publication Detail:
Type:  Journal Article     Date:  2009-06-03
Journal Detail:
Title:  Lipids     Volume:  44     ISSN:  1558-9307     ISO Abbreviation:  Lipids     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-07-02     Completed Date:  2009-09-30     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  621-30     Citation Subset:  IM    
Martek Biosciences Corporation, 6480 Dobbin Rd, Columbia, MD 21045-5825, USA.
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MeSH Terms
Cells, Cultured
Cloning, Molecular
Diatoms / genetics,  metabolism*
Fatty Acid Synthetase Complex, Type II / genetics
Fatty Acids, Unsaturated / biosynthesis*
Lipid Metabolism / genetics*
Metabolic Networks and Pathways / genetics*
Organisms, Genetically Modified
Reg. No./Substance:
0/Fatty Acids, Unsaturated; EC 6.-/Fatty Acid Synthetase Complex, Type II

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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