| Characterization of borderline oxacillin-resistant Staphylococcus aureus isolated from food of animal origin. | |
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MedLine Citation:
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PMID: 20615346 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this study, the molecular characteristics of food-derived oxacillin-resistant Staphylococcus aureus were determined. Eight borderline oxacillin-resistant strains with MICs of 2 to 4 microg/ml were identified from 132 S. aureus isolates of food origin. One of the two isolates with a MIC of 4 microg/ml was methicillin-resistant determinant (mecA) gene positive, and the other six with MICs of 2 microg/ml were mecA negative. The mecA-positive isolate was classified as sequence type (ST)228, staphylococcal protein A (spa) type t041, and carried the staphylococcal cassette chromosome mec type I element. Two borderline oxacillin-resistant strains were classified as spa t008 and ST8, and the remaining five as spa t164 and ST20. The mecA-positive strain and four borderline oxacillin-resistant strains were found enterotoxigenic. The enterotoxin genes detected in these strains included selp, egc1, and sed-sej-selr. The borderline-resistant S. aureus isolates from a manually handled product, i.e., minced pork, were shown genetically related to strains associated with human infections. This suggests that humans can be considered as a source of contamination of this food with oxacillin-resistant S. aureus strains. The genotypes of the investigated milk borderline-resistant isolates were shown to occur not only in cows, but also in humans. Since manual handling is reduced in raw milk production, a human origin of S. aureus seems unlikely. Because knowledge of the genotypes of animal staphylococci is limited, more research is needed to address the question of the origin of antibiotic-resistant S. aureus strains in food. |
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Authors:
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Jarosław Bystroń; Magdalena Podkowik; Kamila Korzekwa; Elzbieta Lis; Jerzy Molenda; Jacek Bania |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of food protection Volume: 73 ISSN: 0362-028X ISO Abbreviation: J. Food Prot. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-07-09 Completed Date: 2010-09-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7703944 Medline TA: J Food Prot Country: United States |
Other Details:
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Languages: eng Pagination: 1325-7 Citation Subset: IM |
Affiliation:
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Department of Food Hygiene and Consumer Health Protection, Wrocław University of Environmental and Life Sciences, Wrocław, Poland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anti-Bacterial Agents / pharmacology* Cattle Colony Count, Microbial Consumer Product Safety Dose-Response Relationship, Drug Drug Resistance, Bacterial* Food Contamination / analysis* Genotype Humans Meat Products / microbiology Microbial Sensitivity Tests Milk / microbiology Oxacillin / pharmacology* Staphylococcus aureus / drug effects*, isolation & purification |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 66-79-5/Oxacillin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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