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Characterization of Bacillus thuringiensis soil isolates from Cuba, with insecticidal activity against mosquitoes.
MedLine Citation:
PMID:  22017108     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
Chemical insecticides may be toxic and cause environmental degradation. Consequently, biological control for insects represents an alternative with low ecological impact. In this work, three soil isolates (A21, A51 and C17) from different regions of the Cuban archipelago were identified, characterized and evaluated against Aedes aegypti and Culex quinquefasciatus. The new isolates were compared with reference IPS82 strain and two strains isolated from biolarvicides Bactivec and Bactoculicida, respectively. The differentiation was done by morphological, biochemical, bioassays activity and molecular methods (SDS-PAGE, plasmid profile and random amplified polymorphic analysis). All isolates were identified as Bacillus thuringiensis. The A21, A51 and C17 isolates showed higher larvicide activity than Bactivec's isolated reference strain, against both A. aegypti and C. quinquefasciatus. A21 isolate had a protein profile similar to IPS82 and Bactivec strain. A51 and C17 isolates produced a characteristic proteins pattern. A21 and A51 isolates had plasmid patterns similar to IPS82 standard strain, while C17 isolate had different both plasmid profile and protein bands. All the studied isolates showed a diverse RAPD patterns and were different from the strains previously used in biological control in Cuba.
Authors:
Aileen González; Raúl Díaz; Manuel Díaz; Yainais Borrero; Rosa Y Bruzón; Bertha Carreras; René Gato
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Revista de biología tropical     Volume:  59     ISSN:  0034-7744     ISO Abbreviation:  Rev. Biol. Trop.     Publication Date:  2011 Sep 
Date Detail:
Created Date:  2011-10-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0404267     Medline TA:  Rev Biol Trop     Country:  Costa Rica    
Other Details:
Languages:  eng     Pagination:  1007-16     Citation Subset:  IM    
Affiliation:
Department of Vector Control, Institute of Tropical Medicine "Pedro Kourí", Havana, PO Box 601, Marianao 13, Ciudad de La Habana, Cuba. aileen@ipk.sld.cu
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