| Characterization of the Bacillus subtilis motile system driven by an artificially created proton motive force. | |
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MedLine Citation:
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PMID: 40954 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Transient swimming was induced in energy-depleted cells of Bacillus subtilis by an artificial proton motive force, which was created by valinomycin addition and a pH reduction. This system did not require any ions except protons in the medium. The size of the induced motility was strongly influenced by changes in the size of either the K+ diffusion potential or the pH gradient. A rough estimation indicated that a proton motive force higher than -100 mV was required for induction of translational swimming of the cell. Corresponding with the transient appearance of swimming, a rapid but transient efflux of K+ and influx of H+ were observed. With decreases in the rate of H+ influx, the amount of motility decreased. A rate of H+ influx higher than 0.2 mumol/s per ml of cell water gave translational swimming. These results suggest direct coupling of H+ influx to rotation of bacterial flagella. |
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Authors:
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S Matsuura; J I Shioi; Y Imae; S Iida |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of bacteriology Volume: 140 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1979 Oct |
Date Detail:
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Created Date: 1980-01-19 Completed Date: 1980-01-19 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 28-36 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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biosynthesis Bacillus subtilis / physiology* Calcium / pharmacology Hydrogen / metabolism* Hydrogen-Ion Concentration Movement Potassium / metabolism* Protons Valinomycin / pharmacology |
| Chemical | |
Reg. No./Substance:
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0/Protons; 1333-74-0/Hydrogen; 2001-95-8/Valinomycin; 56-65-5/Adenosine Triphosphate; 7440-09-7/Potassium; 7440-70-2/Calcium |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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