Document Detail


Characterization of the Bacillus subtilis motile system driven by an artificially created proton motive force.
MedLine Citation:
PMID:  40954     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Transient swimming was induced in energy-depleted cells of Bacillus subtilis by an artificial proton motive force, which was created by valinomycin addition and a pH reduction. This system did not require any ions except protons in the medium. The size of the induced motility was strongly influenced by changes in the size of either the K+ diffusion potential or the pH gradient. A rough estimation indicated that a proton motive force higher than -100 mV was required for induction of translational swimming of the cell. Corresponding with the transient appearance of swimming, a rapid but transient efflux of K+ and influx of H+ were observed. With decreases in the rate of H+ influx, the amount of motility decreased. A rate of H+ influx higher than 0.2 mumol/s per ml of cell water gave translational swimming. These results suggest direct coupling of H+ influx to rotation of bacterial flagella.
Authors:
S Matsuura; J I Shioi; Y Imae; S Iida
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of bacteriology     Volume:  140     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1979 Oct 
Date Detail:
Created Date:  1980-01-19     Completed Date:  1980-01-19     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  28-36     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / biosynthesis
Bacillus subtilis / physiology*
Calcium / pharmacology
Hydrogen / metabolism*
Hydrogen-Ion Concentration
Movement
Potassium / metabolism*
Protons
Valinomycin / pharmacology
Chemical
Reg. No./Substance:
0/Protons; 1333-74-0/Hydrogen; 2001-95-8/Valinomycin; 56-65-5/Adenosine Triphosphate; 7440-09-7/Potassium; 7440-70-2/Calcium
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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