| Characterization of Bacillus halodurans alpha-galactosidase Mel4A encoded by the mel4A gene (BH2228). | |
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MedLine Citation:
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PMID: 18776668 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A family-4 alpha-galactosidase Mel4A of Bacillus halodurans was expressed in Escherichia coli and characterized. Recombinant enzyme rMel4A depended on NAD+, some divalent cations such as Mn2+, and reducing reagents such as dithiothreitol. rMel4A was active on small saccharides such as raffinose but not on highly polymerized galactomannan. Immunological analysis indicated that raffinose induced the production of Mel4A in B. halodurans. |
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Authors:
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Andian Ari Anggraeni; Makiko Sakka; Tetsuya Kimura; Khanok Ratanakhaokchai; Motomitsu Kitaoka; Kazuo Sakka |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-09-07 |
Journal Detail:
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Title: Bioscience, biotechnology, and biochemistry Volume: 72 ISSN: 1347-6947 ISO Abbreviation: Biosci. Biotechnol. Biochem. Publication Date: 2008 Sep |
Date Detail:
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Created Date: 2008-09-24 Completed Date: 2008-12-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9205717 Medline TA: Biosci Biotechnol Biochem Country: Japan |
Other Details:
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Languages: eng Pagination: 2459-62 Citation Subset: IM |
Affiliation:
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Applied Microbiology Laboratory, Graduate School of Bioresources, Mie University, Tsu, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacillus
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enzymology*,
genetics,
metabolism Cations, Divalent / metabolism Dithiothreitol / pharmacology Dose-Response Relationship, Drug Escherichia coli / genetics Genes, Bacterial* Manganese / metabolism Melibiose / genetics* NAD / metabolism, pharmacology Raffinose / metabolism Recombinant Proteins / isolation & purification, metabolism alpha-Galactosidase / genetics*, isolation & purification, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Cations, Divalent; 0/Recombinant Proteins; 3483-12-3/Dithiothreitol; 512-69-6/Raffinose; 53-84-9/NAD; 585-99-9/Melibiose; 7439-96-5/Manganese; EC 3.2.1.22/alpha-Galactosidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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