Document Detail


Characterization of BSA unfolding and aggregation using a single-capillary viscometer and dynamic surface tension detector.
MedLine Citation:
PMID:  21962683     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
A dynamic surface tension detector (DSTD) has been equipped with an additional pressure sensor for simultaneous viscosity measurements, as a detector for flow injection analysis. The viscosity measurement is based on a single capillary viscometer (SCV) placed in parallel configuration with the DSTD. The viscometer in the optimized conditions consists of a PEEK capillary (i.d.=0.25mm, L=75cm) kept at constant temperature using a thermostatic bath, which leads on the two sides to the two arms of a differential piezoelectric pressure transducer with a range of 0-35psi. The DSTD, described previously, measures the changing pressure across the liquid/air interface of 2μL drops repeatedly forming at the end of a capillary. SCV performance has been evaluated by measuring dynamic viscosity of water/glycerol mixtures analysed in flow injection and comparing the results with the values reported in the literature. The detection limits of SCV and DSTD, calculated as 3σ of the blank, were 0.012cP and 0.6dyncm(-1), respectively. The FI-SCV-DSTD system has been applied to the study of temperature-induced denaturation/aggregation process in bovine serum albumin (BSA). The results have been supported and discussed with respect to BSA conformational analysis performed using Fourier Transform infrared spectroscopy.
Authors:
Emilia Bramanti; Carlo Ferrari; Valeria Angeli; Massimo Onor; Robert E Synovec
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Publication Detail:
Type:  Journal Article     Date:  2011-08-12
Journal Detail:
Title:  Talanta     Volume:  85     ISSN:  1873-3573     ISO Abbreviation:  Talanta     Publication Date:  2011 Oct 
Date Detail:
Created Date:  2011-10-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984816R     Medline TA:  Talanta     Country:  England    
Other Details:
Languages:  eng     Pagination:  2553-61     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier B.V. All rights reserved.
Affiliation:
National Research Council of Italy, C.N.R., Istituto di Chimica dei Composti Organo Metallici-ICCOM-UOS Pisa, Area di Ricerca, Via G. Moruzzi 1, 56124 Pisa, Italy.
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