Document Detail


Characterization of Aspergillus nidulans peroxisomes by immunoelectron microscopy.
MedLine Citation:
PMID:  9818356     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In previous work, we have demonstrated that oleate induces a massive proliferation of microbodies (peroxisomes) in Aspergillus nidulans. Although at a lower level, proliferation of peroxisomes also occurs in cells growing under conditions that induce penicillin biosynthesis. Here, microbodies in oleate-grown A. nidulans cells were characterized by using several antibodies that recognize peroxisomal enzymes and peroxins in a broad spectrum of eukaryotic organisms such as yeast, and plant, and mammalian cells. Peroxisomes were immunolabeled by anti-SKL and anti-thiolase antibodies, which suggests that A. nidulans conserves both PTS1 and PTS2 import mechanisms. Isocitrate lyase and malate synthase, the two key enzymes of the glyoxylate cycle, were also localized in these organelles. In contrast to reports of Neurospora crassa, our results demonstrate that A. nidulans contains only one type of microbody (peroxisomes) that carry out the glyoxylate cycle and contain 3-ketoacyl-CoA thiolase and proteins with the C-terminal SKL tripeptide.
Authors:
S Valenciano; J R De Lucas; I Van der Klei; M Veenhuis; F Laborda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of microbiology     Volume:  170     ISSN:  0302-8933     ISO Abbreviation:  Arch. Microbiol.     Publication Date:  1998 Oct 
Date Detail:
Created Date:  1998-12-03     Completed Date:  1998-12-03     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0410427     Medline TA:  Arch Microbiol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  370-6     Citation Subset:  IM    
Affiliation:
Departamento de Microbiología y Parasitología, Facultad de Farmacia, Universidad de Alcalá de Henares, Madrid, Spain.
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MeSH Terms
Descriptor/Qualifier:
Animals
Aspergillus nidulans / enzymology,  growth & development,  ultrastructure*
Blotting, Western
Culture Media
Electrophoresis, Polyacrylamide Gel
Microbodies* / enzymology,  ultrastructure
Microscopy, Immunoelectron
Rabbits
Chemical
Reg. No./Substance:
0/Culture Media

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