Document Detail

Characterization of aromatase binding agents from the dichloromethane extract of Corydalis yanhusuo using ultrafiltration and liquid chromatography tandem mass spectrometry.
MedLine Citation:
PMID:  20657498     Owner:  NLM     Status:  MEDLINE    
Aromatase represents an important target for the treatment of hormone-dependent breast cancer. In the present study, nine alkaloids from the dichloromethane extract of Corydalis yanhusuo were identified by liquid chromatography tandem mass spectrometry (LC-MS/MS) and tested for their aromatase binding activities using an ultrafiltration LC-MS method by investigating the differences of peak areas of compounds before and after incubations with aromatase. It was demonstrated that the quaternary protoberberine alkaloids and the tertiary protoberberine alkaloids exhibited potent aromatase binding activities. The quaternary ammonium group and the methyl group at C-13 position of tertiary protoberberine alkaloids might be necessary for the activity. The findings should provide guidance for the discovery of potential aromatase inhibitors from natural products.
Jing Shi; Xiaoyu Zhang; Zhongjun Ma; Min Zhang; Fang Sun
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-05-14
Journal Detail:
Title:  Molecules (Basel, Switzerland)     Volume:  15     ISSN:  1420-3049     ISO Abbreviation:  Molecules     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-07-26     Completed Date:  2010-11-03     Revised Date:  2010-12-15    
Medline Journal Info:
Nlm Unique ID:  100964009     Medline TA:  Molecules     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  3556-66     Citation Subset:  IM    
Department of Pharmacy, Zhejiang Medical College, No.481 Binwen Rd., Hangzhou 310053, China.
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MeSH Terms
Aromatase / metabolism*
Chromatography, Liquid
Corydalis / chemistry*
Methylene Chloride
Plant Extracts / chemistry*
Protein Binding
Tandem Mass Spectrometry
Reg. No./Substance:
0/Plant Extracts; 75-09-2/Methylene Chloride; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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