| Characterization of aromatase binding agents from the dichloromethane extract of Corydalis yanhusuo using ultrafiltration and liquid chromatography tandem mass spectrometry. | |
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MedLine Citation:
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PMID: 20657498 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Aromatase represents an important target for the treatment of hormone-dependent breast cancer. In the present study, nine alkaloids from the dichloromethane extract of Corydalis yanhusuo were identified by liquid chromatography tandem mass spectrometry (LC-MS/MS) and tested for their aromatase binding activities using an ultrafiltration LC-MS method by investigating the differences of peak areas of compounds before and after incubations with aromatase. It was demonstrated that the quaternary protoberberine alkaloids and the tertiary protoberberine alkaloids exhibited potent aromatase binding activities. The quaternary ammonium group and the methyl group at C-13 position of tertiary protoberberine alkaloids might be necessary for the activity. The findings should provide guidance for the discovery of potential aromatase inhibitors from natural products. |
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Authors:
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Jing Shi; Xiaoyu Zhang; Zhongjun Ma; Min Zhang; Fang Sun |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-05-14 |
Journal Detail:
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Title: Molecules (Basel, Switzerland) Volume: 15 ISSN: 1420-3049 ISO Abbreviation: Molecules Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-07-26 Completed Date: 2010-11-03 Revised Date: 2010-12-15 |
Medline Journal Info:
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Nlm Unique ID: 100964009 Medline TA: Molecules Country: Switzerland |
Other Details:
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Languages: eng Pagination: 3556-66 Citation Subset: IM |
Affiliation:
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Department of Pharmacy, Zhejiang Medical College, No.481 Binwen Rd., Hangzhou 310053, China. shij136@hotmail.com |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aromatase
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metabolism* Chromatography, Liquid Corydalis / chemistry* Methylene Chloride Plant Extracts / chemistry* Protein Binding Tandem Mass Spectrometry Ultrafiltration |
| Chemical | |
Reg. No./Substance:
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0/Plant Extracts; 75-09-2/Methylene Chloride; EC 1.14.14.1/Aromatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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