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Characterization of Arabidopsis thaliana-Fusarium graminearum interactions and identification of variation in resistance among ecotypes.
MedLine Citation:
PMID:  20507455     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
SUMMARY Fusarium graminearum causes fusarium head blight (FHB) of wheat and other cereals. Resistance of wheat to FHB is incomplete and the defence mechanisms involved are poorly understood owing to the complex genome and long life cycle of the host. A robust and reproducible bioassay system was established for the study of interactions between F. graminearum and Arabidopsis by wound inoculation of detached leaves embedded in agar medium. Amendment of the inoculum with deoxynivalenol enhanced symptom development and conidial production. Colonization of leaf tissue was by both inter- and intracellular growth and extensive cell death was observed ahead of advancing hyphae indicating the presence of phytotoxic compounds or host-initiated programmed cell death. Expression of defence response genes associated with jasmonic acid/ethylene and salicylic acid pathways indicated that both pathways were activated in response to F. graminearum with expression of the jasmonic acid/ethylene pathways being more enhanced than that of the salicylic acid pathway, as expected for a necrotrophic pathogen. A large variation in resistance among Arabidopsis ecotypes was revealed. The ecotype Col-0 was significantly more resistant than Ler using both the detached leaf and a detached flower assay. Foliar resistance was shown to be controlled largely by a single genetic factor on chromosome 4 near the RFLP marker O6455. The detached leaf assay described herein provides a means to enable fundamental studies on the defence mechanisms of Arabidopsis in response to F. graminearum.
Authors:
Xinwei Chen; Andrew Steed; Clare Harden; Paul Nicholson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular plant pathology     Volume:  7     ISSN:  1364-3703     ISO Abbreviation:  Mol. Plant Pathol.     Publication Date:  2006 Sep 
Date Detail:
Created Date:  2010-05-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100954969     Medline TA:  Mol Plant Pathol     Country:  England    
Other Details:
Languages:  eng     Pagination:  391-403     Citation Subset:  -    
Affiliation:
John Innes Centre, Norwich Research Park, Colney, Norwich NR4 7UH, UK.
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