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Characterization and Application of Oviductal Epithelial Cells In Vitro in Gallus domesticus.
MedLine Citation:
PMID:  21715713     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Chicken oviductal epithelium massively produces egg white proteins in daily cycles. In this study, we cultured and characterized oviductal cells from juvenile (10-wk-old) chickens and from actively laying (30-wk-old) hens. The juvenile oviduct epithelial cells (OECs) were maintained over passage 25 and were positive for Toluidine Blue, lectin-ConA, -HPA, -UEA-1, -WFA, -WGA, anti-OVA, anti-ESR1, and anti-PGR, whereas the adult OECs were cultured over passage 6 and were positive for Toluidine Blue, periodic acid-Schiff, lectin-ConA, -WFA, -WGA, anti-OVA, anti-ESR1, and anti-PGR. To investigate the optimal concentration of steroid hormones for inducing egg white protein genes in vitro, we examined the effects of estrogen, diethylbesterol, progesterone, and corticosterone on OECs. The results showed that oviduct-specific avidin, ovalbumin, ovomucin, lysozyme, ESR1, and PGR gene expressions were significantly elevated in steroid hormone-treated OECs compared with untreated cells (P < 0.05). Ovalbumin protein was also secreted into the culture media from hormone-treated OECs. In addition, to examine the application of OECs for avian transgenesis, we introduced human thrombopoietin (THPO)-expressing lentiviral vector controlled by a 3.5-kb ovalbumin promoter into cultured OECs, and THPO expression was significantly induced with diethylstilbestrol or progesterone in juvenile OECs (P < 0.05) and in adult OECs (P < 0.05). In conclusion, these data demonstrate the potential of cultured oviductal cells as a model system for providing a better understanding of massive gene expression regulation and for the production of an avian transgenic bioreactor.
Authors:
Jin Gyoung Jung; Tae Sub Park; Jin Nam Kim; Beom Ku Han; Seon Duk Lee; Gwonhwa Song; Jae Yong Han
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-6-29
Journal Detail:
Title:  Biology of reproduction     Volume:  -     ISSN:  1529-7268     ISO Abbreviation:  -     Publication Date:  2011 Jun 
Date Detail:
Created Date:  2011-6-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0207224     Medline TA:  Biol Reprod     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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