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Characterization of Adipose-Derived Mesenchymal Stem Cell Combinations for Vascularized Bone Engineering.
MedLine Citation:
PMID:  23343199     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Since bone repair and regeneration depend on vasculogenesis and osteogenesis, both of these processes are essential for successful vascularized bone engineering. Using adipose-derived stem cells (ASCs), we investigated temporal gene expression profiles, as well as bone nodule and endothelial tubule formation capacities, during osteogenic and vasculogenic ASC lineage commitment. Osteoprogenitor-enriched cell (OPEC) populations were found to express RUNX2, MSX2, SP7 (osterix), BGLAP (osteocalcin), SPARC (osteonectin), and SPP1 (osteopontin) in a temporally specific sequence. Irreversible commitment of ASCs to the osteogenic lineage occurred between days 6 and 9 of differentiation. Endothelioprogenitor-enriched cell (EPEC) populations expressed CD34, PECAM1 (CD31), ENG (CD105), FLT1 (VEGFR1), and KDR (VEGFR2). Capacity for microtubule formation was evident in as early as 3 days. Functional capacity was assessed in eight co-culture combinations for both bone nodule and endothelial tubule formation, and the greatest expression of these end-differentiation phenotypes was observed in the combination of well-differentiated endothelial cells with less-differentiated osteoblastic cells. Taken together, our results demonstrate vascularized bone engineering utilizing ASCs is a promising enterprise, and that co-culture strategies should focus on developing a more mature vascular network in combination with a less mature osteoblastic stromal cell.
Authors:
Cristian D Valenzuela; Alexander C Allori; Derek D Reformat; Alexander M Sailon; Robert J Allen; Edward H Davidson; Mani Alikhani; Timothy G Bromage; John L Ricci; Stephen M Warren
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-24
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  -     ISSN:  1937-335X     ISO Abbreviation:  Tissue Eng Part A     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
New York University Langone Medical Center, Institute of Reconstructive Plastic Surgery Laboratories, 560 First Avenue, TH-169, New York, New York, United States, 10016, 914-844-7305; cristian.valenzuela.md@gmail.com.
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