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Characterization of Acrylamidase Isolated from a Newly Isolated Acrylamide-Utilizing Bacterium, Ralstonia eutropha AUM-01.
MedLine Citation:
PMID:  20872004     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
A mesophilic bacterium capable of utilizing acrylamide was isolated, AUM-01, from soil collected from leaf litter at Picnic Point on the UW-Madison campus. In minimal medium with acrylamide as the sole carbon and nitrogen source, a batch culture of AUM-01 completely converted 28.0 mM acrylamide to acrylic acid in 8 h and reached a cell density of 0.3 (A(600)). Afterward all the acrylic acid was degraded by 20 h with the cell density increasing to 1.9 (A(600)). The acrylamide-utilizing bacterium was identified as Ralstonia eutropha based on morphological observations, the BiOLOG GN2 MicroPlate(TM) identification system for Gram-negative bacteria, and additional physiological tests. An acrylamidase that hydrolyzes acrylamide to acrylic acid was purified from the strain AUM-01. The molecular weight of the enzyme from AUM-01 was determined to be 38 kDa by SDS-PAGE. The enzyme had pH and temperature optima of 6.3 and 55°C, and the influence of different metals and amino acids on the ability of the purified protein to transform acrylamide to acrylic acid was evaluated. The enzyme from AUM-01 was totally inhibited by ZnSO(4) and AgNO(3).
Authors:
Minseok Cha; Glenn H Chambliss
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Publication Detail:
Type:  Journal Article     Date:  2010-09-25
Journal Detail:
Title:  Current microbiology     Volume:  62     ISSN:  1432-0991     ISO Abbreviation:  Curr. Microbiol.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7808448     Medline TA:  Curr Microbiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  671-8     Citation Subset:  IM    
Affiliation:
Department of Bacteriology, University of Wisconsin-Madison, Madison, WI, 53706, USA, Min-Seok.Cha@pnl.gov.
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