Document Detail

Characterization of Acidic Protease from Aspergillus niger BCRC 32720.
MedLine Citation:
PMID:  23286670     Owner:  NLM     Status:  Publisher    
An acid protease from the broth of a 24-hour cultivated Aspergillus niger BCRC 32720 was purified to electrophoretical homogeneity by CM Sepharose FF and Sephacryl S-100 HR chromatographs. The specific activity, purification fold and yield were 23.29 kU/mg, 2.5 and 24.2%, respectively. Molecular mass (M) and N-terminal amino acid sequence were 47.5 kDa and SKGSAVTT, while the pH and temperature optima were at 2.5 and 50(o)C, respectively. It was stable at pH 2.0-4.0 or ≤40(o)C, activated by Fe(2+) and cysteine, but partial inhibited by phenylmethyl-sulfonyl fluoride and tosyllysine chloromethyl ketone and highly inhibited by Ag(+), Sn(2+), Fe(3+), Sb(3+) and pepstatin A. It was considered to be an aspartic protease.
Li-Jung Yin; Tzu-Hui Hsu; Shann-Tzong Jiang
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-3
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  -     ISSN:  1520-5118     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-4     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
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