| Characterization of Acidic Protease from Aspergillus niger BCRC 32720. | |
| | |
MedLine Citation:
|
PMID: 23286670 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
An acid protease from the broth of a 24-hour cultivated Aspergillus niger BCRC 32720 was purified to electrophoretical homogeneity by CM Sepharose FF and Sephacryl S-100 HR chromatographs. The specific activity, purification fold and yield were 23.29 kU/mg, 2.5 and 24.2%, respectively. Molecular mass (M) and N-terminal amino acid sequence were 47.5 kDa and SKGSAVTT, while the pH and temperature optima were at 2.5 and 50(o)C, respectively. It was stable at pH 2.0-4.0 or ≤40(o)C, activated by Fe(2+) and cysteine, but partial inhibited by phenylmethyl-sulfonyl fluoride and tosyllysine chloromethyl ketone and highly inhibited by Ag(+), Sn(2+), Fe(3+), Sb(3+) and pepstatin A. It was considered to be an aspartic protease. |
| | |
Authors:
|
Li-Jung Yin; Tzu-Hui Hsu; Shann-Tzong Jiang |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2013-1-3 |
Journal Detail:
|
Title: Journal of agricultural and food chemistry Volume: - ISSN: 1520-5118 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2013 Jan |
Date Detail:
|
Created Date: 2013-1-4 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The MTA family proteins as novel histone H3 binding proteins.
Next Document: Circulating microRNA signatures in mice exposed to lipoteichoic acid.