Document Detail


Characterization of AMC-HN-9, a cell line established from an undifferentiated carcinoma of the parotid gland: expression of alpha6beta4 with the absence of BP180 and 230.
MedLine Citation:
PMID:  11039880     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We recently reported the development of a cell line, AMC-HN-9, established from an undifferentiated carcinoma (UDC) of the parotid gland. AMC-HN-9 consists mostly of spindle-shaped cells, has poor in vitro adhesiveness and an in vitro appearance that is different from that of other epithelial cell lines. To test the hypothesis that structural or functional abnormalities of the hemidesmosomes might contribute to the morphological appearance and biology of UDCs, we studied the expression of hemidesmosomal proteins in AMC-HN-9. Flow cytometry, indirect immunofluorescence, immunoprecipitation, reverse transcriptase-polymerase chain reaction, and cytogenetic analysis were used. AMC-HN-9 cells express the alpha6 and beta4 integrin subunits at nearly the same intensity as head and neck squamous cell carcinoma cell lines. However, AMC-HN-9 does not express BP180 and BP230, although there is no gross deletion of the loci of the BP180 and BP230 genes, suggesting that a more subtle mechanism has silenced these genes. In conclusion, the failure to express certain hemidesmosomal proteins is a likely explanation for the functional and morphologic characteristics of UDC cells both in vivo and in vitro.
Authors:
S H Kim; T E Carey; M Liebert; S J Yoo; H J Kwon; S Y Kim
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta oto-laryngologica     Volume:  120     ISSN:  0001-6489     ISO Abbreviation:  Acta Otolaryngol.     Publication Date:  2000 Aug 
Date Detail:
Created Date:  2001-02-13     Completed Date:  2001-02-15     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0370354     Medline TA:  Acta Otolaryngol     Country:  NORWAY    
Other Details:
Languages:  eng     Pagination:  660-6     Citation Subset:  IM    
Affiliation:
Department of Clinical Pathology, Samsung Medical Center, University of Sungkyunkwan College of Medicine, Seoul, South Korea,
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal
Antigens, CD / genetics,  metabolism*
Autoantigens / genetics,  metabolism*
Carcinoma / genetics,  metabolism*,  pathology*
Carrier Proteins*
Collagen / genetics,  metabolism*
Cytoskeletal Proteins*
Flow Cytometry
Fluorescent Antibody Technique
Humans
Integrin alpha4
Integrin alpha6
Integrins / genetics,  metabolism*
Karyotyping
Nerve Tissue Proteins*
Non-Fibrillar Collagens*
Parotid Neoplasms / genetics,  metabolism*,  pathology*
Precipitin Tests
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured* / immunology,  metabolism
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, CD; 0/Autoantigens; 0/Carrier Proteins; 0/Cytoskeletal Proteins; 0/DST protein, human; 0/Integrin alpha6; 0/Integrins; 0/Nerve Tissue Proteins; 0/Non-Fibrillar Collagens; 0/collagen type XVII; 143198-26-9/Integrin alpha4; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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