Document Detail


Characterization of AAT1: a gene involved in the regulation of amino acid transport in Saccharomyces cerevisiae.
MedLine Citation:
PMID:  2697749     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A new class of Saccharomyces cerevisiae mutants (aat1 - amino acid transport) has been identified. These mutants are unable to grow on rich medium or on minimal medium supplemented with certain amino acids (isoleucine, methionine, phenylalanine, tyrosine or valine). This phenotype is directly linked to the presence of the leu2 allele in these strains: aat1 LEU2 organisms grow normally on all media tested. Leucine uptake through the leucine-specific permease is inhibited to less than 35% of wild-type levels in aat1 cells preincubated in nonpermissive media, and the activity of the general amino acid permease is also low in these conditions. aat1 cells are therefore unable to grow on rich media because they cannot take up enough leucine to supplement their auxotrophic requirement.
Authors:
J M Garrett
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of general microbiology     Volume:  135     ISSN:  0022-1287     ISO Abbreviation:  J. Gen. Microbiol.     Publication Date:  1989 Sep 
Date Detail:
Created Date:  1990-04-26     Completed Date:  1990-04-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0375371     Medline TA:  J Gen Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2429-37     Citation Subset:  IM    
Affiliation:
Department of Biology, Hamilton College, Clinton, New York 13323.
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / pharmacokinetics*
Biological Transport / physiology
Culture Media
Genes, Fungal / physiology*
Leucine / pharmacokinetics
Mutation*
Phenotype
Saccharomyces cerevisiae / genetics*,  growth & development,  metabolism
Grant Support
ID/Acronym/Agency:
GM37441/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Culture Media; 61-90-5/Leucine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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