Document Detail

Characterization of 3-hydroxyisobutyrate dehydrogenase, HIBADH, as a sperm-motility marker.
MedLine Citation:
PMID:  23423614     Owner:  NLM     Status:  Publisher    
PURPOSE: Asthenozoospermia is a major cause of male infertility. However, the molecular mechanisms underlying sperm-motility defects remain largely unknown in the majority of cases. In our previous study, we applied a proteomic approach to identify unknown proteins that were downregulated in spermatozoa with low motility compared to spermatozoa with good motility. Several sperm motility- related proteins have been identified. In this study, 3-hydroxyisobutyrate dehydrogenase (HIBADH), one of the proteins identified using the proteomic tools, is further characterized. METHODS: Reverse-transcription polymerase chain reactions (RT-PCR), western blotting, and immunofluorescence assays (IFA) were preformed to investigate the expression pattern. The enzymatic activity of HIBADH was evaluated in sperm with good (>50 %), moderate (< 50 %) and lower motility (< 20 %). RESULTS: Using RT-PCR, we found that transcripts of HIBADH are enriched in the cerebellum, heart, skeletal muscle, uterus, placenta, and testes of male humans. In western blotting, it is expressed in the placenta, testes, and spermatozoa. During spermiogenesis, HIBADH is located at the mid-piece (a specialized development from the mitochondria) of elongating, elongated, and mature sperm. The enzymatic activity of HIBADH in sperm with moderate and lower motility were significantly reduced compared with good motility (P < 0.0001 and P < 0.05, respectively). CONCLUSIONS: Our study indicated that HIBADH is involved in the mitochondrial function of spermatozoa, and maintains sperm motility. It may serve as a sperm-motility marker.
Yung-Chieh Tasi; Hsin-Chih Albert Chao; Chia-Ling Chung; Xiu-Ying Liu; Ying-Ming Lin; Pao-Chi Liao; Hsien-An Pan; Han-Sun Chiang; Pao-Lin Kuo; Ying-Hung Lin
Related Documents :
23504054 - Rcb20, an experimental benzimidazole derivative, affects tubulin expression and induces...
460184 - Regulation of albumin synthesis in rat liver.
10651994 - Ultraviolet-b exposure of human skin induces cytochromes p450 1a1 and 1b1.
7505254 - Complement regulatory proteins in early human fetal life: cd59, membrane co-factor prot...
10766854 - Mrna and protein stability regulate the differential expression of pro- and anti-inflam...
10859354 - Exploring the sequence space for tetracycline-dependent transcriptional activators: nov...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-2-20
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  -     ISSN:  1573-7330     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-2-20     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Obstetrics and Gynecology, Chi-Mei Medical Center, Tainan, Taiwan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Oocyte maturation and in vitro hormone production in small antral follicles (SAFs) isolated from rhe...
Next Document:  Correlation between Endoscopic and Histological Diagnoses of Gastric Intestinal Metaplasia.