Document Detail

Characteristics of a two-site immunoradiometric assay for human skeletal alkaline phosphatase in serum.
MedLine Citation:
PMID:  8174258     Owner:  NLM     Status:  MEDLINE    
This two-site IRMA includes specific monoclonal antibodies for measuring skeletal alkaline phosphatase (B-ALP) in human serum. Assay calibration is based on mass units (micrograms per liter) and was established with purified B-ALP from a human osteosarcoma cell line, SAOS-2. Precision studies demonstrated intra- and interassay CVs of 3-5% and 5-7%, respectively. Relative reactivity studies showed that the assay has a sevenfold preference for detecting B-ALP compared with the liver isoenzyme in serum. The normal reference interval for 478 healthy adults was 5-22 micrograms/L. Method comparison studies showed good correlation between this B-ALP assay (y) and commercially available electrophoretic methods (x) (y = 0.3540x + 20.5, R2 = 0.929) in a pagetic population. Temporal profiles for total ALP, this IRMA B-ALP assay, and B-ALP by electrophoresis in three pagetic patients were parallel. We conclude that this assay demonstrates good analytical performance and would be useful for the clinical assessment of metabolic bone disorders.
K Panigrahi; P D Delmas; F Singer; W Ryan; O Reiss; R Fisher; P D Miller; I Mizrahi; C Darte; B C Kress
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical chemistry     Volume:  40     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1994 May 
Date Detail:
Created Date:  1994-06-08     Completed Date:  1994-06-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  822-8     Citation Subset:  IM    
Department of Pathology, University of Maryland, School of Medicine, Baltimore 21201.
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MeSH Terms
Aged, 80 and over
Alkaline Phosphatase / blood*,  chemistry
Amino Acids / analysis
Bone and Bones / enzymology*
Enzyme Stability
Immunoradiometric Assay / methods*,  statistics & numerical data
Isoenzymes / blood*,  chemistry
Liver / enzymology
Middle Aged
Osteitis Deformans / enzymology
Reference Values
Sensitivity and Specificity
Tumor Cells, Cultured
Reg. No./Substance:
0/Amino Acids; 0/Isoenzymes; EC Phosphatase

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