Document Detail


Characteristics of a two-site immunoradiometric assay for human skeletal alkaline phosphatase in serum.
MedLine Citation:
PMID:  8174258     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This two-site IRMA includes specific monoclonal antibodies for measuring skeletal alkaline phosphatase (B-ALP) in human serum. Assay calibration is based on mass units (micrograms per liter) and was established with purified B-ALP from a human osteosarcoma cell line, SAOS-2. Precision studies demonstrated intra- and interassay CVs of 3-5% and 5-7%, respectively. Relative reactivity studies showed that the assay has a sevenfold preference for detecting B-ALP compared with the liver isoenzyme in serum. The normal reference interval for 478 healthy adults was 5-22 micrograms/L. Method comparison studies showed good correlation between this B-ALP assay (y) and commercially available electrophoretic methods (x) (y = 0.3540x + 20.5, R2 = 0.929) in a pagetic population. Temporal profiles for total ALP, this IRMA B-ALP assay, and B-ALP by electrophoresis in three pagetic patients were parallel. We conclude that this assay demonstrates good analytical performance and would be useful for the clinical assessment of metabolic bone disorders.
Authors:
K Panigrahi; P D Delmas; F Singer; W Ryan; O Reiss; R Fisher; P D Miller; I Mizrahi; C Darte; B C Kress
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical chemistry     Volume:  40     ISSN:  0009-9147     ISO Abbreviation:  Clin. Chem.     Publication Date:  1994 May 
Date Detail:
Created Date:  1994-06-08     Completed Date:  1994-06-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9421549     Medline TA:  Clin Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  822-8     Citation Subset:  IM    
Affiliation:
Department of Pathology, University of Maryland, School of Medicine, Baltimore 21201.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aged
Aged, 80 and over
Alkaline Phosphatase / blood*,  chemistry
Amino Acids / analysis
Bone and Bones / enzymology*
Electrophoresis
Enzyme Stability
Female
Humans
Immunoradiometric Assay / methods*,  statistics & numerical data
Isoenzymes / blood*,  chemistry
Liver / enzymology
Male
Middle Aged
Osteitis Deformans / enzymology
Osteosarcoma
Reference Values
Sensitivity and Specificity
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Isoenzymes; EC 3.1.3.1/Alkaline Phosphatase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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