| Characteristics of revertants induced by EMS and u.v. light from a 6-thioguanine resistant HGPRT deficient V79 Chinese hamster cell line. | |
| | |
MedLine Citation:
|
PMID: 22282999 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
A spontaneous TG(R) HAT(s) V79 mutant had an HGPRT specific activity of less than 5% that of the wild type. Only two spontaneous revertants were recovered from more than 10(8) mutant cells screened. However, HAT resistant clones were recovered, after EMS mutagenesis at a frequency of 4.5 x 10(-6) and at a lower frequency from u.v. irradiated mutant cells. Of more than 400 clones tested, over 90% had regained a HAT(R) TG(s) HGPRT(+) revertant phenotype. Nine of these revertants that were further characterised had HGPRT specific activities within the wild type range and showed wild type electrophoretic mobility. These data suggest that the original HGPRT(-) phenotype was of a mutational rather than an adaptational origin. |
| | |
Authors:
|
R J Hodgkiss; M Fox |
Related Documents
:
|
9245789 - Dictyostelium rasg is required for normal motility and cytokinesis, but not growth. 7493629 - Motility of vinculin-deficient f9 embryonic carcinoma cells analyzed by video, laser co... 11693299 - Early nephron formation in the developing mouse kidney. 11428129 - Spore coat formation and timely sporulation depend on cellulose in dictyostelium. 22704979 - The emergence and maintenance of sickle cell hotspots in the mediterranean. 9440539 - Myxococcus xanthus displays frz-dependent chemokinetic behavior during vegetative swarm... 15784889 - Expression of a toxoneuron nigriceps polydnavirus-encoded protein causes apoptosis-like... 1165439 - Calcitonin production by rat thyroid tumours. 18098349 - Branched fungal beta-glucan causes hyperinflammation and necrosis in phagocyte nadph ox... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Carcinogenesis Volume: 1 ISSN: 0143-3334 ISO Abbreviation: Carcinogenesis Publication Date: 1980 Feb |
Date Detail:
|
Created Date: 2012-01-30 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8008055 Medline TA: Carcinogenesis Country: England |
Other Details:
|
Languages: eng Pagination: 189-98 Citation Subset: IM |
Affiliation:
|
Paterson Laboratories, Christie Hospital and Holt Radium Institute, Manchester M20 9BX, UK. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
| Grant Support | |
ID/Acronym/Agency:
|
//Medical Research Council |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Reversion of 6-thioguanine resistant Chinese hamster cell lines: agent specificity and evidence for ...
Next Document: Effects of temporary selenium supplementation on the genesis of spontaneous mammary tumors in inbred...