Document Detail

Characteristics of progenitor cells derived from adult ciliary body in mouse, rat, and human eyes.
MedLine Citation:
PMID:  17389499     Owner:  NLM     Status:  MEDLINE    
PURPOSE: To isolate and characterize progenitor cells derived from adult mammalian ciliary body.
METHODS: The authors isolated progenitor cells from the ciliary body of adult mice, rats, and human cadaver eyes and determined quantitative growth characteristics of groups of progenitor cells called neurosphere (NS) cells, including individual cell diameter, NS diameter, percentage of NS-forming cells, and cell number per eye in mouse, rat, and human eyes. The immunolabeling and ultrastructure of NS cells were investigated by confocal and transmission electron microscopy.
RESULTS: Average diameters of individual cells and neurospheres after 1 week in culture were similar in mice, rats, and humans (cell diameters: 22 +/- 1.1, 21 +/- 0.3, 25 +/- 0.4 mum; NS diameters: 139 +/- 22, 137 +/- 9, 141 +/- 11 mum, respectively). Mean numbers of cells per NS were estimated to be 1183 in mice, 5360 in rats, and 685 in humans. Molecules that were identified by immunolabeling in NS cells included nestin, Chx-10, vimentin, GFAP, and Pax-6. Thy-1 was expressed in some NS cells. Ultrastructurally, NS cells displayed abundant rough endoplasmic reticulum and many cellular processes but no characteristics of mature retinal neurons or glia.
CONCLUSIONS: Progenitor cells from adult mammalian ciliary body have significant, but limited, proliferation potential and express markers characteristic of other progenitor cells and seen during early retinal development. The ciliary body could be a source of cells for transplantation in experimental rodent eyes and for autotransplantation in human eyes.
Hangxiu Xu; Drina D Sta Iglesia; Jennifer L Kielczewski; Danielle F Valenta; Mary E Pease; Donald J Zack; Harry A Quigley
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  48     ISSN:  0146-0404     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2007 Apr 
Date Detail:
Created Date:  2007-03-28     Completed Date:  2007-05-14     Revised Date:  2011-10-26    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1674-82     Citation Subset:  IM    
Glaucoma Research Laboratory, Wilmer Eye Institute, Johns Hopkins University School of Medicine, 600 North Wolfe Street, Baltimore, MD 21287, USA.
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MeSH Terms
Aged, 80 and over
Antigens, Thy-1 / metabolism
Biological Markers / metabolism
Cell Count
Cell Culture Techniques
Cell Proliferation
Cell Separation
Ciliary Body / cytology*,  physiology
Eye Proteins / metabolism
Glial Fibrillary Acidic Protein / metabolism
Homeodomain Proteins / metabolism
Intermediate Filament Proteins / metabolism
Mice, Inbred C57BL
Microscopy, Confocal
Microscopy, Electron, Transmission
Nerve Tissue Proteins / metabolism
Paired Box Transcription Factors / metabolism
Rats, Wistar
Repressor Proteins / metabolism
Stem Cells / cytology*,  physiology
Transcription Factors / metabolism
Vimentin / metabolism
Grant Support
P30 EY 001765/EY/NEI NIH HHS
Reg. No./Substance:
0/Antigens, Thy-1; 0/Biological Markers; 0/Eye Proteins; 0/Glial Fibrillary Acidic Protein; 0/Homeodomain Proteins; 0/Intermediate Filament Proteins; 0/Nerve Tissue Proteins; 0/PAX6 protein; 0/Paired Box Transcription Factors; 0/Repressor Proteins; 0/Transcription Factors; 0/VSX2 protein, human; 0/Vimentin; 0/Vsx2 protein, mouse; 0/nestin

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