| Characteristics of a metastatic variant to the liver of human rectal adenocarcinoma cell line RCM-1. | |
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MedLine Citation:
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PMID: 8407210 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The interaction of human rectal adenocarcinoma cell line RCM-1 cells with extracellular matrix components, was studied to elucidate the key steps in the liver metastasis of colorectal carcinomas. Highly metastatic variant L-10 cells selected from the metastatic foci of the liver after intrasplenic implantation in nude mice and its parental L-0 cells were used. L-10 cells showed a greater ability to adhere to laminin, fibronectin, and type I and type IV collagens than did L-0 cells but less haptotactic activity than that of L-0 cells to type I or type IV collagen, possibly due to the formation of cellular aggregates. In vitro invasion activities of both cell lines to basement membrane components (Matrigel) or type I collagen were minimal but enhanced by the addition of 12-O-tetradecanoylphorbol-13-acetate (TPA). L-10 cells showed greater ability to invade Matrigel than did L-0 cells, while L-0 cells exhibited higher activity in the invasion of type I collagen than did L-10 cells. TPA did not increase the production of metalloproteinases by both cells when analyzed by gelatin zymography. Based on the differences between the two cell lines, we postulated the following: (1) the high metastatic potential of L-10 cells was due to a greater capacity to attach to and cross the basement membrane; (2) TPA directly enhanced tumor cell invasiveness, not via the increased secretion of metalloproteinases; and (3) haptotactic migration had no significant correlation with the increased metastatic potential of L-10 cells. |
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Authors:
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N Komada; K Nabeshima; H Koita; H Kataoka; K Muraoka; M Koono |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Invasion & metastasis Volume: 13 ISSN: 0251-1789 ISO Abbreviation: Invasion Metastasis Publication Date: 1993 |
Date Detail:
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Created Date: 1993-11-01 Completed Date: 1993-11-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8202435 Medline TA: Invasion Metastasis Country: SWITZERLAND |
Other Details:
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Languages: eng Pagination: 38-49 Citation Subset: IM |
Affiliation:
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Department of Pathology, Miyazaki Medical College, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenocarcinoma
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enzymology,
secondary* Animals Cell Adhesion / physiology Cell Aggregation / physiology Cell Movement / physiology Extracellular Matrix / metabolism Extracellular Matrix Proteins / metabolism Humans Liver Neoplasms / secondary* Metalloendopeptidases / metabolism Mice Mice, Inbred BALB C Mice, Nude Neoplasm Invasiveness / physiopathology Rectal Neoplasms / enzymology, pathology* Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Extracellular Matrix Proteins; EC 3.4.24.-/Metalloendopeptidases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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