Document Detail


Characteristics of a metastatic variant to the liver of human rectal adenocarcinoma cell line RCM-1.
MedLine Citation:
PMID:  8407210     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The interaction of human rectal adenocarcinoma cell line RCM-1 cells with extracellular matrix components, was studied to elucidate the key steps in the liver metastasis of colorectal carcinomas. Highly metastatic variant L-10 cells selected from the metastatic foci of the liver after intrasplenic implantation in nude mice and its parental L-0 cells were used. L-10 cells showed a greater ability to adhere to laminin, fibronectin, and type I and type IV collagens than did L-0 cells but less haptotactic activity than that of L-0 cells to type I or type IV collagen, possibly due to the formation of cellular aggregates. In vitro invasion activities of both cell lines to basement membrane components (Matrigel) or type I collagen were minimal but enhanced by the addition of 12-O-tetradecanoylphorbol-13-acetate (TPA). L-10 cells showed greater ability to invade Matrigel than did L-0 cells, while L-0 cells exhibited higher activity in the invasion of type I collagen than did L-10 cells. TPA did not increase the production of metalloproteinases by both cells when analyzed by gelatin zymography. Based on the differences between the two cell lines, we postulated the following: (1) the high metastatic potential of L-10 cells was due to a greater capacity to attach to and cross the basement membrane; (2) TPA directly enhanced tumor cell invasiveness, not via the increased secretion of metalloproteinases; and (3) haptotactic migration had no significant correlation with the increased metastatic potential of L-10 cells.
Authors:
N Komada; K Nabeshima; H Koita; H Kataoka; K Muraoka; M Koono
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Invasion & metastasis     Volume:  13     ISSN:  0251-1789     ISO Abbreviation:  Invasion Metastasis     Publication Date:  1993  
Date Detail:
Created Date:  1993-11-01     Completed Date:  1993-11-01     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8202435     Medline TA:  Invasion Metastasis     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  38-49     Citation Subset:  IM    
Affiliation:
Department of Pathology, Miyazaki Medical College, Japan.
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MeSH Terms
Descriptor/Qualifier:
Adenocarcinoma / enzymology,  secondary*
Animals
Cell Adhesion / physiology
Cell Aggregation / physiology
Cell Movement / physiology
Extracellular Matrix / metabolism
Extracellular Matrix Proteins / metabolism
Humans
Liver Neoplasms / secondary*
Metalloendopeptidases / metabolism
Mice
Mice, Inbred BALB C
Mice, Nude
Neoplasm Invasiveness / physiopathology
Rectal Neoplasms / enzymology,  pathology*
Tumor Cells, Cultured
Chemical
Reg. No./Substance:
0/Extracellular Matrix Proteins; EC 3.4.24.-/Metalloendopeptidases

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