| Characteristics of intestinal lamina propria dendritic cells in a mouse model of postinfectious irritable bowel syndrome. | |
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MedLine Citation:
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PMID: 22141367 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Background and Aims: Postinfectious irritable bowel syndrome (PI-IBS) which results from inflammation has been emphasized a lot recently. Dendritic cell (DC) may contribute to intestinal mucosal immune activation in the pathogenesis of PI-IBS. This study tested the hypothesis that phenotype and function of intestinal lamina propria DCs (LPDCs) changed in the development of PI-IBS mouse model. Methods: Mice infected with T. spiralis underwent abdominal withdrawal reflex (AWR) to evaluate visceral sensitivity. LPDCs were isolated and purified by intestine digestion and magnetic label-based technique. Surface markers were analyzed by flow cytometry. Endocytic activity, mixed lymphocyte reaction (MLR) and chemotaxis were studied. Cytokine production of the LPDCs cocultured with CD4(+) T cells was determined. Results: Intestinal inflammation resolved after 8 weeks infection with sustained visceral hyperalgesia. Surface markers CD86 and MHCII were lower in the acute infection group, but increased in the PI-IBS stage. Enhanced ability of endocytic activity and decreased abilities to attract and stimulate CD4(+) T cell proliferation were in acute infection group. However, LPDCs in PI-IBS stage showed weakened endocytic ability with enhanced abilities to attract and stimulate CD4(+) T cell proliferation. Cocultured LPDCs with CD4(+) T cells showed a predominant Th2 response in the acute infection stage, and more important roles of Th1, Th17 responses in the PI-IBS stage. Conclusions: The hypothesis was supported that the phenotype and function of LPDCs changed in the development of PI-IBS, which induced the maintenance of intestinal mucosal immune activation and might provide a clue for the treatment of the disease. |
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Authors:
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Yanqin Long; Wenfeng Wang; Huan Wang; Liangcheng Hao; Wei Qian; Xiaohua Hou |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-12-6 |
Journal Detail:
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Title: Journal of gastroenterology and hepatology Volume: - ISSN: 1440-1746 ISO Abbreviation: - Publication Date: 2011 Dec |
Date Detail:
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Created Date: 2011-12-6 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8607909 Medline TA: J Gastroenterol Hepatol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2011 Journal of Gastroenterology and Hepatology Foundation and Blackwell Publishing Asia Pty Ltd. |
Affiliation:
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Division of Gastroenterology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, 1277,Jiefang Road, Wuhan, China. 430022. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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