Document Detail


Characteristics of NADH-dependent lipid peroxidation in sarcoplasmic reticulum of white shrimp, Litopenaeus vannamei, and freshwater prawn, Macrobrachium rosenbergii.
MedLine Citation:
PMID:  12892760     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The iron-catalyzed NADH-dependent lipid peroxidation system in sarcoplasmic reticulum (SR) of cultured white shrimp, Litopenaeus vannamei and freshwater prawn, Macrobrachium rosenbergii was characterized. Production of thiobarbituric acid reactive substances was used to measure the activity of lipid peroxidation. In both species, the system preferred NADH to NADPH as the reducing agent. Lipid peroxidation activities of SR from both species increased when reaction temperatures increased from 6 to 26 degrees C. At 66 degrees C, the reaction was no longer NADH-dependent. Acidic pH amplified the lipid peroxidation activity. Sarcoplasmic reticular lipid peroxidation activity in white shrimp was always greater than in freshwater prawn. Fatty acid composition of SR lipids could be a major factor for this outcome. The proportion of n-3 highly unsaturated fatty acids, such as C20:5 and C22:6, in sarcoplasmic reticular lipids of white shrimp was twice of that in freshwater prawn. The results of this study provide important tools required for anti-oxidative nutrient study at sub-cellular level.
Authors:
Yu-Ruh Lin; Sue-Lan Huang; Chen-Huei Huang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology     Volume:  135     ISSN:  1096-4959     ISO Abbreviation:  Comp. Biochem. Physiol. B, Biochem. Mol. Biol.     Publication Date:  2003 Aug 
Date Detail:
Created Date:  2003-08-01     Completed Date:  2004-04-22     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9516061     Medline TA:  Comp Biochem Physiol B Biochem Mol Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  683-7     Citation Subset:  IM    
Affiliation:
National Chiayi University, Department of Aquatic Biosciences, 300 University Road, Chiayi 600, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Hydrogen-Ion Concentration
Lipid Metabolism
Lipid Peroxidation*
Lipids / chemistry
NAD / metabolism*
Palaemonidae / anatomy & histology,  metabolism*
Penaeidae / anatomy & histology,  metabolism*
Sarcoplasmic Reticulum / chemistry,  metabolism*
Temperature
Thiobarbituric Acid Reactive Substances / metabolism
Chemical
Reg. No./Substance:
0/Lipids; 0/Thiobarbituric Acid Reactive Substances; 53-84-9/NAD

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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