| Characteristics of High-Molecular-Weight Hyaluronic Acid as a Brain-Derived Neurotrophic Factor Scaffold in Periodontal Tissue Regeneration. | |
| | |
MedLine Citation:
|
PMID: 21091323 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Brain-derived neurotrophic factor (BDNF), for which bovine collagen-derived atelocollagen is used as a scaffold, enhances periodontal tissue regeneration. However, a scaffold that does not contain unknown ingredients is preferable. Since the synthesized high-molecular-weight (HMW)-hyaluronic acid (HA) is safe and inexpensive, we evaluated the efficacy of HMW-HA as a BDNF scaffold. CD44, a major receptor of HA, was expressed in cultures of human periodontal ligament cells, and HMW-HA promoted the adhesion and proliferation of human periodontal ligament cells, although it did not influence the mRNA expression of bone (cementum)-related proteins. The in vitro release kinetics of BDNF from HMW-HA showed that BDNF release was sustained for 14 days. Subsequently, we examined the effect of BDNF/HMW-HA complex on periodontal tissue regeneration in dogs. A greater volume of newly formed alveolar bone and a longer newly formed cementum were observed in the BDNF/HMW-HA group than in the HMW-HA group, suggesting that HMW-HA assists the regenerative capacity of BDNF, although HMW-HA itself does not enhance periodontal tissue regeneration. Neither the poly (lactic-co-glycolic acid) group nor the BDNF/poly (lactic-co-glycolic acid) group enhanced periodontal tissue regeneration. In conclusion, HMW-HA is an adequate scaffold for the clinical application of BDNF. |
| | |
Authors:
|
Katsuhiro Takeda; Noriyuki Sakai; Hideki Shiba; Takayoshi Nagahara; Tsuyoshi Fujita; Mikihito Kajiya; Tomoyuki Iwata; Shinji Matsuda; Kazuko Kawahara; Hiroyuki Kawaguchi; Hidemi Kurihara |
Related Documents
:
|
10036143 - Bovine serum albumin-(7-hydroxycoumarin-4-acetic acid) complex: applications to the flu... 10359023 - Fatty acid desaturation: effect of alphafetoprotein on alpha-linolenic acid conversion ... 10036143 - Bovine serum albumin-(7-hydroxycoumarin-4-acetic acid) complex: applications to the flu... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-1-16 |
Journal Detail:
|
Title: Tissue engineering. Part A Volume: - ISSN: 1937-335X ISO Abbreviation: - Publication Date: 2011 Jan |
Date Detail:
|
Created Date: 2011-1-17 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101466659 Medline TA: Tissue Eng Part A Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
|
This work was performed at the Division of Frontier Medical Science, Department of Periodontal Medicine, Hiroshima University Graduate School of Biomedical Sciences , Hiroshima, Japan . |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Mechanisms of gender-specific regulation of mouse sulfotransferases (Sults).
Next Document: Effects of 1 GeV/nucleon (56)Fe Particles on Longevity, Carcinogenesis and Neuromotor Ability in Atm...