Document Detail


Characteristics of HeLa strains: permanent vs. variable features.
MedLine Citation:
PMID:  7002488     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Characteristic rearranged human chromosome markers have been observed in a variety of HeLa cell sublines and in five suspected HeLa contaminant lines originally thought to be derived from differentiated tissues of different individual patients. The allozyme genetic signatures, representing the composite enzyme phenotype at eight polymorphic loci, of each of the studied contaminant lines were identical to each other and to those of HeLa cells. The probability that each of these lines would have an identical genetic signature (since the frequency of the HeLa genotype is 0.0017) is 4.2 X 10(-15). Differences between cell lines, however, could be detected by isoelectric focussing of the isoenzymes for glucose-6-phosphate dehydrogenase. The cell lines, including CLL 74, the older Chang liver line, failed to express five liver-specific proteins. One protein was detected in a new liver cell culture. Variations in cytogenetic, biochemical, and differentiated functions during continuous cell culture are discussed.
Authors:
W A Nelson-Rees; L Hunter; G J Darlington; S J O'Brien
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Cytogenetics and cell genetics     Volume:  27     ISSN:  0301-0171     ISO Abbreviation:  Cytogenet. Cell Genet.     Publication Date:  1980  
Date Detail:
Created Date:  1981-02-26     Completed Date:  1981-02-26     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0367735     Medline TA:  Cytogenet Cell Genet     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  216-31     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Cell Differentiation
Cell Line
Chromosome Banding
Genetic Markers*
Hela Cells* / enzymology
Humans
Karyotyping
Phenotype
Chemical
Reg. No./Substance:
0/Genetic Markers

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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