Document Detail


Characteristics of cultured tomato cells after prolonged exposure to medium containing polyethylene glycol.
MedLine Citation:
PMID:  16662239     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Cell lines of tomato (Lycopersicon esculentum Mill., cv. VFNT-Cherry) have been isolated, which are capable of growing in media containing polyethylene glycol (PEG) 6000 with water potentials as low as -15 or -22 bar. After prolonged exposure to media containing PEG, these cell populations have reduced abilities to grow in the absence of PEG. Upon resuspension in PEG-free medium, the cells swell and begin to release metabolites, including protein. Measurement by plasmometry of the osmotic potential of cells selected in medium with -22 bar water potential indicates that they maintain, at the end of the growth cycle, an osmotic potential of approximately -26 bar. This is compared to an osmotic potential of -9 bar for nonselected cells in medium without PEG, having an initial water potential of -4 bar. Thus, considerable osmotic adjustment occurs as a result of exposure to external low water potential. The results also indicate that PEG does not contribute significantly to osmotic adjustment of the cells.
Authors:
A K Handa; R A Bressan; S Handa; P M Hasegawa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plant physiology     Volume:  69     ISSN:  0032-0889     ISO Abbreviation:  Plant Physiol.     Publication Date:  1982 Feb 
Date Detail:
Created Date:  2010-06-29     Completed Date:  2010-06-29     Revised Date:  2010-09-15    
Medline Journal Info:
Nlm Unique ID:  0401224     Medline TA:  Plant Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  514-21     Citation Subset:  -    
Affiliation:
Department of Horticulture, Purdue University, West Lafayette, Indiana 47907.
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