Document Detail


Characteristics of 3H-cis-flupenthixol binding in rat striatum.
MedLine Citation:
PMID:  6645796     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Characteristics of membrane receptor binding by 3H-cis-flupenthixol were examined in rat striatum. Using modifications of standard dopamine receptor binding techniques, it was possible to obtain 70% specific binding with 3H-cis-flupenthixol. Association and dissociation rates were very rapid, with equilibrium reached in 2 min and half-time of dissociation being 1 min. Analysis of saturation and competition studies using cis-flupenthixol and spiroperidol indicated that cis-flupenthixol binds to two striatal receptors with apparent KD's of 0.7 and 4.8 nM. It is suggested these represent D1 and D2 dopamine receptors respectively. The further characterization of the properties of cis-flupenthixol binding presented here should enable more detailed studies of multiple dopamine receptors to be designed.
Authors:
L C Murrin
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Life sciences     Volume:  33     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:  1983 Nov 
Date Detail:
Created Date:  1984-01-26     Completed Date:  1984-01-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  2179-86     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Binding Sites
Corpus Striatum / metabolism*
Flupenthixol / metabolism*
Kinetics
Polysorbates / pharmacology
Rats
Rats, Inbred Strains
Receptors, Dopamine / metabolism
Thioxanthenes / metabolism*
Tritium / diagnostic use
Chemical
Reg. No./Substance:
0/Polysorbates; 0/Receptors, Dopamine; 0/Thioxanthenes; 10028-17-8/Tritium; 2709-56-0/Flupenthixol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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