Document Detail

Characteristic cellular composition of germinal centers.
MedLine Citation:
PMID:  18291526     Owner:  NLM     Status:  MEDLINE    
We established the method of isolating individually encapsulated germinal centers (GCs) from immunized spleen and analyzed single cell suspension of GCs by flowcytometry. In GCs, the high frequency of sIgG+ cells (29%) and sIgA+ cells (5%) was detected. Two-color flowcytometry analysis showed that GCs contained 27% of sIgM-IgG+ cells, in which isotype switch from IgM to IgG had occurred, and 5% of Bu1-IgG+ cells, which were differentiating into plasma cells. On the other hand, sIgM-IgG+ and Bu1-IgG+ cells were not detected in the bursa, which contained 95% of B cells and only 1% of T cells. CD4+ but not CD8+ T cells were detected in the light zone of GCs and these CD4+ T cells are supposed to play a key role in isotype switching and differentiation into plasma cells in GCs. These results clearly demonstrate that GCs provide a site for isotype switching and differentiation into plasma cells.
Keiko Imamura; Masahiro Yasuda; Brigitte Riwar; Seiji Inui; Shigeo Ekino
Related Documents :
24561166 - Live-cell fluorescent microscopy platforms for real-time monitoring of polyplex-cell in...
24805866 - Role and mechanism of twist1 in modulating the chemosensitivity of fadu cells.
1164126 - Plasma cells in urine: occurrence in multiple myeloma.
22925926 - High rate of antibody secretion is not integral to plasma cell differentiation as revea...
16897566 - Brood-cell size does not influence the susceptibility of honey bees (apis mellifera) to...
7752526 - Cftr and other cl- channels in human airway cells.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-04
Journal Detail:
Title:  Comparative immunology, microbiology and infectious diseases     Volume:  32     ISSN:  1878-1667     ISO Abbreviation:  Comp. Immunol. Microbiol. Infect. Dis.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-04-06     Completed Date:  2009-06-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7808924     Medline TA:  Comp Immunol Microbiol Infect Dis     Country:  England    
Other Details:
Languages:  eng     Pagination:  419-28     Citation Subset:  IM    
Department of Histology, Graduate School of Medical Sciences, Kumamoto University, Honjo, Kumamoto, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
B-Lymphocyte Subsets / immunology*,  metabolism
Cell Differentiation / immunology*
Chickens / immunology
Germinal Center / cytology,  immunology*,  metabolism
Immunoglobulin Class Switching*
Immunoglobulins / immunology,  metabolism
Plasma Cells / immunology,  metabolism
Spleen / immunology,  metabolism
T-Lymphocyte Subsets / immunology*,  metabolism
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Transient pancytopenia preceding acute lymphoblastic leukemia with positive Philadelphia chromosome.
Next Document:  Assessment of ambient air quality in Eskişehir, Turkey.