Document Detail

Characteristic cellular composition of germinal centers.
MedLine Citation:
PMID:  18291526     Owner:  NLM     Status:  MEDLINE    
We established the method of isolating individually encapsulated germinal centers (GCs) from immunized spleen and analyzed single cell suspension of GCs by flowcytometry. In GCs, the high frequency of sIgG+ cells (29%) and sIgA+ cells (5%) was detected. Two-color flowcytometry analysis showed that GCs contained 27% of sIgM-IgG+ cells, in which isotype switch from IgM to IgG had occurred, and 5% of Bu1-IgG+ cells, which were differentiating into plasma cells. On the other hand, sIgM-IgG+ and Bu1-IgG+ cells were not detected in the bursa, which contained 95% of B cells and only 1% of T cells. CD4+ but not CD8+ T cells were detected in the light zone of GCs and these CD4+ T cells are supposed to play a key role in isotype switching and differentiation into plasma cells in GCs. These results clearly demonstrate that GCs provide a site for isotype switching and differentiation into plasma cells.
Keiko Imamura; Masahiro Yasuda; Brigitte Riwar; Seiji Inui; Shigeo Ekino
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-04
Journal Detail:
Title:  Comparative immunology, microbiology and infectious diseases     Volume:  32     ISSN:  1878-1667     ISO Abbreviation:  Comp. Immunol. Microbiol. Infect. Dis.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-04-06     Completed Date:  2009-06-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7808924     Medline TA:  Comp Immunol Microbiol Infect Dis     Country:  England    
Other Details:
Languages:  eng     Pagination:  419-28     Citation Subset:  IM    
Department of Histology, Graduate School of Medical Sciences, Kumamoto University, Honjo, Kumamoto, Japan.
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MeSH Terms
B-Lymphocyte Subsets / immunology*,  metabolism
Cell Differentiation / immunology*
Chickens / immunology
Germinal Center / cytology,  immunology*,  metabolism
Immunoglobulin Class Switching*
Immunoglobulins / immunology,  metabolism
Plasma Cells / immunology,  metabolism
Spleen / immunology,  metabolism
T-Lymphocyte Subsets / immunology*,  metabolism
Reg. No./Substance:

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