Document Detail


Characteristic cellular composition of germinal centers.
MedLine Citation:
PMID:  18291526     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We established the method of isolating individually encapsulated germinal centers (GCs) from immunized spleen and analyzed single cell suspension of GCs by flowcytometry. In GCs, the high frequency of sIgG+ cells (29%) and sIgA+ cells (5%) was detected. Two-color flowcytometry analysis showed that GCs contained 27% of sIgM-IgG+ cells, in which isotype switch from IgM to IgG had occurred, and 5% of Bu1-IgG+ cells, which were differentiating into plasma cells. On the other hand, sIgM-IgG+ and Bu1-IgG+ cells were not detected in the bursa, which contained 95% of B cells and only 1% of T cells. CD4+ but not CD8+ T cells were detected in the light zone of GCs and these CD4+ T cells are supposed to play a key role in isotype switching and differentiation into plasma cells in GCs. These results clearly demonstrate that GCs provide a site for isotype switching and differentiation into plasma cells.
Authors:
Keiko Imamura; Masahiro Yasuda; Brigitte Riwar; Seiji Inui; Shigeo Ekino
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-04
Journal Detail:
Title:  Comparative immunology, microbiology and infectious diseases     Volume:  32     ISSN:  1878-1667     ISO Abbreviation:  Comp. Immunol. Microbiol. Infect. Dis.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-04-06     Completed Date:  2009-06-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7808924     Medline TA:  Comp Immunol Microbiol Infect Dis     Country:  England    
Other Details:
Languages:  eng     Pagination:  419-28     Citation Subset:  IM    
Affiliation:
Department of Histology, Graduate School of Medical Sciences, Kumamoto University, Honjo, Kumamoto, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
B-Lymphocyte Subsets / immunology*,  metabolism
Cell Differentiation / immunology*
Chickens / immunology
Germinal Center / cytology,  immunology*,  metabolism
Immunoglobulin Class Switching*
Immunoglobulins / immunology,  metabolism
Plasma Cells / immunology,  metabolism
Spleen / immunology,  metabolism
T-Lymphocyte Subsets / immunology*,  metabolism
Chemical
Reg. No./Substance:
0/Immunoglobulins

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