| Characteristic cellular composition of germinal centers. | |
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MedLine Citation:
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PMID: 18291526 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We established the method of isolating individually encapsulated germinal centers (GCs) from immunized spleen and analyzed single cell suspension of GCs by flowcytometry. In GCs, the high frequency of sIgG+ cells (29%) and sIgA+ cells (5%) was detected. Two-color flowcytometry analysis showed that GCs contained 27% of sIgM-IgG+ cells, in which isotype switch from IgM to IgG had occurred, and 5% of Bu1-IgG+ cells, which were differentiating into plasma cells. On the other hand, sIgM-IgG+ and Bu1-IgG+ cells were not detected in the bursa, which contained 95% of B cells and only 1% of T cells. CD4+ but not CD8+ T cells were detected in the light zone of GCs and these CD4+ T cells are supposed to play a key role in isotype switching and differentiation into plasma cells in GCs. These results clearly demonstrate that GCs provide a site for isotype switching and differentiation into plasma cells. |
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Authors:
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Keiko Imamura; Masahiro Yasuda; Brigitte Riwar; Seiji Inui; Shigeo Ekino |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-03-04 |
Journal Detail:
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Title: Comparative immunology, microbiology and infectious diseases Volume: 32 ISSN: 1878-1667 ISO Abbreviation: Comp. Immunol. Microbiol. Infect. Dis. Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-04-06 Completed Date: 2009-06-05 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7808924 Medline TA: Comp Immunol Microbiol Infect Dis Country: England |
Other Details:
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Languages: eng Pagination: 419-28 Citation Subset: IM |
Affiliation:
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Department of Histology, Graduate School of Medical Sciences, Kumamoto University, Honjo, Kumamoto, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals B-Lymphocyte Subsets / immunology*, metabolism Cell Differentiation / immunology* Chickens / immunology Germinal Center / cytology, immunology*, metabolism Immunoglobulin Class Switching* Immunoglobulins / immunology, metabolism Plasma Cells / immunology, metabolism Spleen / immunology, metabolism T-Lymphocyte Subsets / immunology*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Immunoglobulins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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