Document Detail


Characterisation of a new isolate of Mycobacterium shimoidei from Finland.
MedLine Citation:
PMID:  11023191     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This report describes the first isolation of Mycobacterium shimoidei in Finland from a sputum specimen obtained from an elderly female patient. M. shimoidei, a potential lung pathogen, is difficult to identify by routine methods and only a few cases have been reported. The present study demonstrated that M. shimoidei has a characteristic pattern for fatty acids and alcohols in gas liquid chromatography. This chromatogram and the pattern of mycolic acids on thin-layer chromatography allow it to be distinguished routinely. The unique sequence of the 16S rRNA gene and the 16S-23S rDNA spacer region allows identification by molecular methods.
Authors:
P Koukila-Kähkölä; L Paulin; E Brander; E Jantzen; M Eho-Remes; M L Katila
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of medical microbiology     Volume:  49     ISSN:  0022-2615     ISO Abbreviation:  J. Med. Microbiol.     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2000-10-19     Completed Date:  2000-10-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0224131     Medline TA:  J Med Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  937-40     Citation Subset:  IM    
Affiliation:
Institute of Biotechnology, University of Helsinki, Finland. pirkko.koukila-kahkola@huch.fi
Data Bank Information
Bank Name/Acc. No.:
GENBANK/AJ005005
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MeSH Terms
Descriptor/Qualifier:
Aged
Base Sequence
Chromatography, Gas
Chromatography, Thin Layer
DNA, Ribosomal / chemistry
Fatty Acids / analysis
Fatty Alcohols / analysis
Female
Finland
Humans
Molecular Sequence Data
Mycobacterium / chemistry*,  classification,  genetics
Mycobacterium Infections / microbiology*,  pathology
Mycolic Acids / analysis
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
Sputum / microbiology
Chemical
Reg. No./Substance:
0/DNA, Ribosomal; 0/Fatty Acids; 0/Fatty Alcohols; 0/Mycolic Acids; 0/RNA, Ribosomal, 16S

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