Document Detail


Characterisation of microtubule-associated proteins at the synapse: absence of MAP 2.
MedLine Citation:
PMID:  11596488     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The high molecular weight microtubule-associated proteins MAP 1 and MAP 2 are major components of brain cytosol and can be readily identified using polyacrylamide gel electrophoresis on the basis of heat-stability and co-sedimentation with microtubules. An examination of synaptosomal cytosol, synaptic plasma membrane and postsynaptic density fractions showed that MAP 2 is absent from these fractions and thus from both pre- and postsynaptic sites. All of the fractions contained polypeptides that comigrated with MAP 1 and a MAP 1 like polypeptide was identified in a microtubule preparation from synaptosomal cytosol. The absence of MAP 2 from synaptosomal cytosol was confirmed by immunoblotting using an antibody directed against MAP 2. Immunocytochemistry using this antibody showed that MAP 2 was present in cell bodies and dendrites but absent from axons.
Authors:
R D Burgoyne; R Cumming
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of cell biology     Volume:  30     ISSN:  0171-9335     ISO Abbreviation:  Eur. J. Cell Biol.     Publication Date:  1983 May 
Date Detail:
Created Date:  2001-10-12     Completed Date:  2001-11-01     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  7906240     Medline TA:  Eur J Cell Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  154-8     Citation Subset:  IM    
Affiliation:
Laboratory of Biological Ultrastructure, National Institute for Medical Research, Mill Hill, London NW7 1AA/United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Animals
Axons / metabolism
Brain / metabolism
Cell Membrane / metabolism
Cytosol / metabolism
Dendrites / metabolism
Electrophoresis, Polyacrylamide Gel
Immunoblotting
Immunohistochemistry
Male
Microtubule-Associated Proteins / biosynthesis*
Microtubules / chemistry*,  metabolism*
Neurons / metabolism
Peptides / chemistry
Rats
Rats, Wistar
Synapses / metabolism*
Synaptosomes / metabolism
Chemical
Reg. No./Substance:
0/Microtubule-Associated Proteins; 0/Peptides

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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