| Characterisation of the microbial diversity in a pig manure storage pit using small subunit rDNA sequence analysis. | |
| | |
MedLine Citation:
|
PMID: 16329909 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The microbial community structure of pig manure slurry (PMS) was determined with comparative analysis of 202 bacterial, 44 archaeal and 33 eukaryotic small subunit (SSU) rDNA partial sequences. Based on a criterion of 97% of sequence similarity, the phylogenetic analyses revealed a total of 108, eight and five phylotypes for the Bacteria, Archaea and Eukarya lineages, respectively. Only 36% of the bacterial phylotypes were closely related (>or=97% similarity) to any previously known sequence in databases. The bacterial groups most often represented in terms of phylotype and clone abundance were the Eubacterium (22% of total sequences), the Clostridium (15% of sequences), the Bacillus-Lactobacillus-Streptococcus subdivision (20% of sequences), theMycoplasma and relatives (10% of sequences) and the Flexibacter-Cytophaga-Bacteroides (20% of sequences). The global microbial community structure and phylotype diversity show a close relationship to the pig gastrointestinal tract ecosystem whereas phylotypes from the Acholeplasma-Anaeroplasma and the Clostridium purinolyticum groups appear to be better represented in manure. Archaeal diversity was dominated by three phylotypes clustering with a group of uncultured microorganisms of unknown activity and only distantly related to the Thermoplasmales and relatives. Other Archaea were methanogenic H2/CO2 utilisers. No known acetoclastic Archaea methanogen was found. Eukaryotic diversity was represented by a pluricellular nematode, two Alveolata, a Blastocystis and an Entamoebidae. Manure slurry physico-chemical characteristics were analysed. Possible inhibitory effects of acetate, sulphide and ammonia concentrations on the microbial anaerobic ecosystem are discussed. |
| | |
Authors:
|
Ra?l Snell-Castro; Jean-Jacques Godon; Jean-Philippe Delgen?s; Patrick Dabert |
Related Documents
:
|
20183519 - Molecular biological characterization and biostimulation of ammonia-oxidizing bacteria ... 15946289 - Niche heterogeneity determines bacterial community structure in the termite gut (reticu... 12957939 - Filamentous "epsilonproteobacteria" dominate microbial mats from sulfidic cave springs. 22851789 - Resistance to change and preference for variable versus fixed response sequences. 10543789 - Direct analysis of genes encoding 16s rrna from complex communities reveals many novel ... 18826439 - Subsurface microbiology and biogeochemistry of a deep, cold-water carbonate mound from ... 22607639 - Development of a novel pcr assay based on the 16s-23s rrna internal transcribed spacer ... 11144219 - Cluster of genes encoding the major egg envelope protein of zebrafish. 1634529 - A native rna secondary structure controls alternative splice-site selection and generat... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2004-12-25 |
Journal Detail:
|
Title: FEMS microbiology ecology Volume: 52 ISSN: 0168-6496 ISO Abbreviation: FEMS Microbiol. Ecol. Publication Date: 2005 Apr |
Date Detail:
|
Created Date: 2005-12-06 Completed Date: 2005-12-30 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 8901229 Medline TA: FEMS Microbiol Ecol Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 229-42 Citation Subset: IM |
Affiliation:
|
Laboratoire de Biotechnologie de l'Environnement, Institut National de la Recherche Agronomique, Avenue des Etangs, 11100 Narbonne, France. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Archaea / genetics, isolation & purification Bacteria / genetics*, isolation & purification* Biodiversity* DNA, Bacterial / analysis*, genetics DNA, Ribosomal / analysis, genetics* Eukaryota / genetics, isolation & purification Manure / microbiology* Medical Waste Disposal Phylogeny Swine / microbiology* |
| Chemical | |
Reg. No./Substance:
|
0/DNA, Bacterial; 0/DNA, Ribosomal; 0/Manure; 0/Medical Waste Disposal |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effects of the humic substances of de-inking paper sludge on the antagonism between two compost bact...
Next Document: On-board flow cytometric observation of picoplankton community structure in the East China Sea durin...