Document Detail

Characterisation of the lysosomal heterogeneity in Chinese-hamster fibroblasts.
MedLine Citation:
PMID:  949969     Owner:  NLM     Status:  MEDLINE    
1. Homogenates of Chinese hamster fibroblasts have been fractionated by analytical centrifugation techniques. Lysosomes have been characterised and identified by the distinctive behaviour of specific marker constituents. 2. The acid hydrolases of hamster fibroblasts were located in two distinct organelles, characterised by differences in their equilibrium densities and rates of sedimentation. The lighter population of lysosomes with a modal density of 1.10 g cm-3, contained glycosidases, nucleases and acid phosphatase. The denser population, with a modal density of 1.15 g cm-3, contained proteases and arylsulphatase B. 3. Acid hydrolases in both populations showed similar degrees of latency and structure-linked sedimentability. 4. The heterophagic activity of hamster fibroblasts was investigated by growing the cells in the presence of [14C]sucrose, [14C]inulin and Triton WR 1339. These compounds are taken up by the cells and appear to be located in the denser population of lysosomes. 5. The low density of the lighter population of lysosomes appears to result from a high content of cholesterol esters and triacylglycerols. It is suggested that the lysosomes may be the site of storage of a considerable proportion of these lipids in cultured hamster cells.
M J Warburton; C H Wynn
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of biochemistry / FEBS     Volume:  65     ISSN:  0014-2956     ISO Abbreviation:  Eur. J. Biochem.     Publication Date:  1976 Jun 
Date Detail:
Created Date:  1976-10-02     Completed Date:  1976-10-02     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  0107600     Medline TA:  Eur J Biochem     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  341-8     Citation Subset:  IM    
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MeSH Terms
Acetylglucosaminidase / metabolism
Acid Phosphatase / metabolism
Cathepsins / metabolism
Cells, Cultured
Centrifugation, Density Gradient
Chondro-4-Sulfatase / metabolism
Glucuronidase / metabolism
Hydrolases / metabolism*
Lipid Metabolism
Lysosomes / enzymology*
Polyethylene Glycols / metabolism
Reg. No./Substance:
0/Polyethylene Glycols; EC 3.-/Hydrolases; EC Phosphatase; EC; EC; EC; EC 3.4.-/Cathepsins

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