Document Detail


Characterisation of lipoxygenase isoforms from olive callus cultures.
MedLine Citation:
PMID:  18790508     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Two lipoxygenase isoforms from olive callus cultures were separated from each other. Acetone powders were made to stabilise activity and remove lipids. Separation was then achieved by salt precipitation and ion-exchange chromatography. Both isoforms had comparable activity with linoleic and alpha-linolenic acid substrates, a basic pH optimum and had molecular masses of around 95 kDa. The callus extracts preferentially formed the 13-hydroperoxy products, in keeping with the pattern of volatile derivatives found in olive tissues and oils derived therefrom.
Authors:
Mark Williams; John L Harwood
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-09-13
Journal Detail:
Title:  Phytochemistry     Volume:  69     ISSN:  0031-9422     ISO Abbreviation:  Phytochemistry     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-12-08     Completed Date:  2009-01-16     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0151434     Medline TA:  Phytochemistry     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2532-8     Citation Subset:  IM    
Affiliation:
School of Biosciences, Cardiff University, Cardiff CF10 3AX, Wales, UK.
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MeSH Terms
Descriptor/Qualifier:
Cells, Cultured
Chromatography, Ion Exchange
Hydrogen-Ion Concentration
Isoenzymes
Linoleic Acid / metabolism
Lipoxygenase / isolation & purification*,  metabolism*
Molecular Weight
Olea / enzymology*
Substrate Specificity
alpha-Linolenic Acid / metabolism
Chemical
Reg. No./Substance:
0/Isoenzymes; 2197-37-7/Linoleic Acid; 463-40-1/alpha-Linolenic Acid; EC 1.13.11.12/Lipoxygenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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