Document Detail

Characterisation of liposomes containing aminolevulinic acid and derived esters.
MedLine Citation:
PMID:  18468913     Owner:  NLM     Status:  MEDLINE    
Liposomes of different compositions have been designed to improve delivery of aminolevulinic acid (ALA) and its esterified derivatives ALA-Hexyl ester (He-ALA) and ALA-Undecanoyl ester (Und-ALA) for its use in photodynamic therapy (PDT). Egg yolk phosphatidyl choline (PC), phosphatidic acid (PA) and phosphatidyl glycerol (PG) were employed in the preparation of the liposomes. Sonicated vesicles composed of PC, PC-PG (80:20) or PC-PA (80:20) containing ALA or derivatives were obtained and purified by a minicolumn centrifugation method. PC liposomes presented encapsulation percentages around 6% for 2 mM ALA, 13% for 2 mM He-ALA and 51% for 2 mM Und-ALA. The addition of PG or PA to the formulation, resulted in an increased entrapment: 19% for 2 mM ALA, 69% for 2 mM He-ALA and 87% for 2 mM Und-ALA in PC-PG liposomes and 21% for 2 mM ALA, 60% for 2 mM He-ALA and 87% for 2 mM Und-ALA in PC-PA liposomes. Higher concentrations of ALA or derivatives resulted in lower percentages of entrapment. The three formulations containing ALA or derivatives were stable up to 1 week upon storage at 4 degrees C. However, upon dilution with medium, ALA leaked from the liposomes, while on the contrary, He-ALA was highly retained, being therefore a good choice for its use in PDT. The stability of Und-ALA upon dilution could not be tested, but Und-ALA proved to have the highest entrapment efficacy.
Gabriela Di Venosa; Laura Hermida; Alcira Batlle; Haydée Fukuda; María Victoria Defain; Leandro Mamone; Lorena Rodriguez; Alexander MacRobert; Adriana Casas
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-04-07
Journal Detail:
Title:  Journal of photochemistry and photobiology. B, Biology     Volume:  92     ISSN:  1011-1344     ISO Abbreviation:  J. Photochem. Photobiol. B, Biol.     Publication Date:  2008 Jul 
Date Detail:
Created Date:  2008-07-14     Completed Date:  2008-10-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8804966     Medline TA:  J Photochem Photobiol B     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  1-9     Citation Subset:  IM    
Centro de Investigaciones sobre Porfirinas y Porfirias, CONICET and Hospital de Clínicas José de San Martín, University of Buenos Aires, Córdoba 2351 1er subsuelo,Ciudad de Buenos Aires, CP 1120AAF, Argentina.
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MeSH Terms
Aminolevulinic Acid / analogs & derivatives*,  analysis*
Drug Delivery Systems*
Esters / analysis
Liposomes / chemistry*,  isolation & purification
Phosphatidic Acids
Photochemotherapy / methods
Grant Support
067062/Z/029/Z//Wellcome Trust
Reg. No./Substance:
0/Esters; 0/Liposomes; 0/Phosphatidic Acids; 0/Phosphatidylcholines; 0/Phosphatidylglycerols; 106-60-5/Aminolevulinic Acid

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