| Characterisation of in vivo ovarian cancer models by quantitative (1) H magnetic resonance spectroscopy and diffusion-weighted imaging. | |
| | |
MedLine Citation:
|
PMID: 22020805 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Magnetic resonance imaging (MRI) and spectroscopy (MRS) offer powerful approaches for detecting physiological and metabolic alterations in malignancies and help investigate underlying molecular mechanisms. Research on epithelial ovarian carcinoma (EOC), the gynaecological malignancy with the highest death rate characterised by frequent relapse and onset of drug resistance, could benefit from application of these molecular imaging approaches. In this study, MRI/MRS were used to characterise solid tumour models obtained by subcutaneous (s.c.) or intraperitoneal (i.p.) implantation of human SKOV3.ip cells in severe combined immunodeficiency (SCID) mice. In vivo MRI/MRS, ex vivo magic-angle-spinning (MAS), and in vitro (1) H-NMR measurements were carried out at 4.7 T, 9.4 T, and 9.4/16.5 T, respectively. MRI evaluation was performed by T1-, T2-, and diffusion-weighted (DW) multislice spin-echo imaging. The in vivo (1) H spectra of all tumour models showed a prominent resonance of total choline-containing metabolites (tCho). Quantitative in vivo MRS of both i.p. and s.c. SKOV3.ip xenografts showed that the mean tCho content was in the 2.9-4.5 mM range, with a mean PCho/tCho ratio of 0.99 ± 0.01 [23 examinations, 14-34 days post injection (dpi)], in good agreement with ex vivo and in vitro analyses. Myo-inositol ranged between 11.7 and 17.0 mM, with a trend towards higher values in i.p. xenografts at 14-16 dpi. The average apparent diffusion coefficient (ADC) values of SKOV3.ip xenografts [1.64 ± 0.11 (n = 9, i.p.) and 1.58 ± 0.03 x10(-3) mm(2) /s (n = 7, s.c.)] were in agreement with values reported for tumours from patients with EOC, while the mean vascular signal fraction (VSF) was lower (≤ 4%), probably due to the more rapid growth of preclinical models. Both s.c. and i.p. xenografts are valuable preclinical models for monitoring biochemical and physiopathological changes associated with in vivo EOC tumour growth and response to therapy, which may serve as the basis for further clinical development of noninvasive MR approaches. Copyright © 2011 John Wiley & Sons, Ltd. |
| | |
Authors:
|
Rossella Canese; Maria Elena Pisanu; Delia Mezzanzanica; Alessandro Ricci; Luisa Paris; Marina Bagnoli; Barbara Valeri; Massimo Spada; Massimo Venditti; Albino Cesolini; Andrea Rodomonte; Massimo Giannini; Silvana Canevari; Franca Podo; Egidio Iorio |
Related Documents
:
|
16202135 - Systematic review and meta-analysis of the diagnostic accuracy of ultrasonography for d... 18029905 - Radiologic diagnosis of cerebral venous thrombosis: pictorial review. 8662065 - Complex congenital heart malformation evaluated with mr imaging at 0.3 t. 8273695 - Portal vein thrombosis: imaging findings. 12195615 - Spontaneous intracranial hypotension in the absence of magnetic resonance imaging abnor... 3944315 - Ct identification of cortical speech areas in the human brain. |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2011-10-24 |
Journal Detail:
|
Title: NMR in biomedicine Volume: - ISSN: 1099-1492 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
|
Created Date: 2011-10-24 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8915233 Medline TA: NMR Biomed Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
|
Copyright © 2011 John Wiley & Sons, Ltd. |
Affiliation:
|
Department of Cell Biology and Neurosciences, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Combination of PAGE and LA-ICP-MS as an analytical workflow in metallomics: state of the art, new qu...
Next Document: Emodin inhibits the differentiation and maturation of dendritic cells and increases the ...