Document Detail


Characterisation of extracellular polysaccharides produced by Crypthecodinium cohnii.
MedLine Citation:
PMID:  11759692     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The valuable polyunsaturated fatty acid, docosahexaenoic acid, can be produced by cultivation of the heterotrophic microalga, Crypthecodinium cohnii. During batch growth of C. cohnii on glucose, sea salt and yeast extract for 5 days, so far unreported extracellular polysaccharides were produced. These caused an increased viscosity and a strong drop in the maximum oxygen transfer. The viscosity increased most markedly as cells entered the stationary phase. The polysaccharides varied in size (from 6 kDa to >1,660 kDa) and monomer distribution. A high molecular mass fraction (from 100 kDa to >1,660 kDa) and a medium molecular mass fraction (6-48 kDa) were prepared. The high molecular mass fraction contained (on a molar basis) 71.7% glucose, 13.1% galactose and 3.8% mannose, whereas the medium molecular mass fraction contained 37.7% glucose, 19.8% galactose and 28.1% mannose. Other monomers present in both fractions were fucose, uronic acid and xylose. Monomers were coupled mainly via alpha-(1-3) links. Increased viscosity due to polysaccharide production complicates the development of commercial, high cell-density processes for the production of docosahexaenoic acid.
Authors:
M E de Swaaf; G J Grobben; G Eggink; T C de Rijk; P van der Meer; L Sijtsma
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied microbiology and biotechnology     Volume:  57     ISSN:  0175-7598     ISO Abbreviation:  Appl. Microbiol. Biotechnol.     Publication Date:  2001 Oct 
Date Detail:
Created Date:  2001-12-10     Completed Date:  2002-05-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8406612     Medline TA:  Appl Microbiol Biotechnol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  395-400     Citation Subset:  IM    
Affiliation:
Agrotechnological Research Institute (ATO), Wageningen, The Netherlands. M.E.deSwaaf@ato.wag-ur.nl
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromatography, High Pressure Liquid
Dinoflagellida / growth & development,  metabolism*
Docosahexaenoic Acids / metabolism
Electrophoresis, Capillary
Glucose / analysis,  metabolism
Molecular Weight
Polysaccharides / biosynthesis*,  chemistry
Viscosity
Chemical
Reg. No./Substance:
0/Polysaccharides; 25167-62-8/Docosahexaenoic Acids; 50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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