| Characterisation of the enzymatic 4-O-acetylation of sialic acids in microsomes from equine submandibular glands. | |
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MedLine Citation:
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PMID: 11511809 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Microsomes prepared from equine submandibular glands and incubated with tritium-labelled AcCoA incorporated acid-insoluble radioactivity in a manner dependent on time, protein, membrane integrity and AcCoA concentration, with incorporation being optimal at 37 degrees C and pH 6.6. Under the experimental conditions used a K(M) of 32.1 microM for AcCoA and a V(max) of 1.2 pmol/mg protein x min was obtained. The incorporation of acid-insoluble radioactivity was also inhibited by CoA in a competitive manner (K(i)=240 microM), as well as by para-chloromercuribenzoate, 3'-dephospho-CoA, 5'-IDP, 5'-ADP, beta-NAD and 4,4'-diisothiocyanatostilbene-2,2'-disulfonate. We demonstrate here that this incorporation of radioactivity into endogenous sialic acid is due to the action of an AcCoA:sialate-4-O-acetyltransferase [EC 2.3.1.44]. Radio thin-layer chromatography analyses of propionic acid-released sialic acids showed that the incorporation of radioactivity correlated with the formation of a radiolabelled species that co-migrated with authentic Neu4,5Ac2. Saponification experiments using NaOH, mouse hepatitis virus strain S and Influenza C/JJ/50 virus also showed that the transfer of [3H]acetyl groups from [3H]AcCoA to endogenous sialic acid acceptors was occurring exclusively at carbon 4 of the pyranose ring. |
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Authors:
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J Tiralongo; H Schmid; R Thun; M Iwersen; R Schauer |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Glycoconjugate journal Volume: 17 ISSN: 0282-0080 ISO Abbreviation: Glycoconj. J. Publication Date: 2000 Dec |
Date Detail:
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Created Date: 2001-08-20 Completed Date: 2002-01-03 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8603310 Medline TA: Glycoconj J Country: United States |
Other Details:
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Languages: eng Pagination: 849-58 Citation Subset: IM |
Affiliation:
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Biochemisches Institut, Christian-Albrechts-Universität, Olshausenstr. 40, D-24098 Kiel, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acetyl Coenzyme A
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metabolism Acetylation Animals Cattle Chromatography, High Pressure Liquid Chromatography, Thin Layer Fluorescence Horses* Hydrogen-Ion Concentration Kinetics Microsomes / chemistry, enzymology, metabolism Radiography Sialic Acids / analysis, metabolism* Submandibular Gland / chemistry, enzymology, metabolism* Temperature |
| Chemical | |
Reg. No./Substance:
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0/Sialic Acids; 72-89-9/Acetyl Coenzyme A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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