Document Detail


Characterisation of castor oil by on-line and off-line non-aqueous reverse-phase high-performance liquid chromatography-mass spectrometry (APCI and UV/MALDI).
MedLine Citation:
PMID:  14667059     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A non-aqueous reverse-phase HPLC method, based on two columns in series, has been used to separate the major triacylglycerols (TAGs) from commercial castor oil and to perform either on-line negative ion atmospheric pressure chemical ionisation (APCI), or off-line positive ion matrix-assisted laser desorption ionisation (MALDI)/MS. The resulting Mass Spectra showed chloride-attached TAG molecules [M + CI]- in the case of negative-ion APCI, and sodium-attached TAG molecules [M + Na]+ in the case of positive-ion MALDI. For MALDI time-of-flight (TOF)/MS, a liquid binary matrix system consisting of sodium ferrocyanide and glycerol was applied, resulting in excellent TAG sensitivity, which was necessary for the determination of trace amounts of TAGs in castor oil. Both techniques allowed unambiguous molecular mass determination of the intact TAG molecules with no thermal degradation. Furthermore, seamless post source decay (PSD) fragment ion analysis by means of a curved field reflector TOF mass spectrometer allowed the determination of the fatty acid composition of each individual TAG. Castor oil contained eight different TAGs which were successfully determined by both APCI and MALDI techniques. In each TAG, at least two units of 12-hydroxy-9-octadecenoic acid (ricinoleic acid) were present. The following fatty acids were determined by seamless PSD fragment ion analysis and APCI/MALDI molecular mass determination as TAG substructures: ricinoleic acid, palmitic acid, stearic acid, oleic acid, linoleic acid, linolenic acid, dihydroxy stearic acid and eicosenoic acid. Triricinolein was the dominating TAG.
Authors:
Gerald Stübiger; Ernst Pittenauer; Günter Allmaier
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Phytochemical analysis : PCA     Volume:  14     ISSN:  0958-0344     ISO Abbreviation:  Phytochem Anal     Publication Date:    2003 Nov-Dec
Date Detail:
Created Date:  2003-12-11     Completed Date:  2004-02-24     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9200492     Medline TA:  Phytochem Anal     Country:  England    
Other Details:
Languages:  eng     Pagination:  337-46     Citation Subset:  IM    
Affiliation:
Institute of Chemical Technologies and Analysis, Vienna University of Technology, Getreidemarkt 9/164, A-1060 Vienna, Austria.
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MeSH Terms
Descriptor/Qualifier:
Castor Oil / analysis,  chemistry*
Chromatography, High Pressure Liquid
Fatty Acids / analysis
Molecular Weight
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Triglycerides / analysis*
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Triglycerides; 8001-79-4/Castor Oil

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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