Document Detail


Characterisation of the bacterial community in expressed prostatic secretions from patients with chronic prostatitis/chronic pelvic pain syndrome and infertile men: a preliminary investigation.
MedLine Citation:
PMID:  22635162     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The expressed prostatic secretions (EPSs) of men with chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS), infertile men and normal men were subjected to microbiological study. EPSs were collected from the subjects, which included 26 normal men, 11 infertile patients and 51 CP/CPPS patients. DNA was extracted from each specimen, and the V3 regions of the 16S rRNA genes were amplified using universal bacterial primers. The results showed that the EPS 16S rRNA gene-positive rate in the CP/CPPS and infertile patients was much higher than in the normal men, but without any difference among the three patient groups. The denaturing gradient gel electrophoresis (DGGE) method was used to characterize the EPS bacterial community structure of the prostate fluid from patients with CP/CPPS or infertility issues. Principal component analysis (PCA) and partial least squares (PLS) analyses of PCR-DGGE profiles revealed that the EPS bacterial community structure differed among the three groups. Three bands were identified as the key factors responsible for the discrepancy between CP/CPPS patients and infertile patients (P<0.05). Two bands were identified as priority factors in the discrepancy of category IIIA and category IIIB prostatitis patients (P<0.05). According to this research, the ecological balance of the prostate and low urethra tract, when considered as a microenvironment, might play an important role in the maintenance of a healthy male reproductive tract.
Authors:
Dong-Sheng Hou; Wen-Min Long; Jian Shen; Li-Ping Zhao; Xiao-Yan Pang; Chen Xu
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-05-28
Journal Detail:
Title:  Asian journal of andrology     Volume:  14     ISSN:  1745-7262     ISO Abbreviation:  Asian J. Androl.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-07-04     Completed Date:  2012-12-21     Revised Date:  2013-09-17    
Medline Journal Info:
Nlm Unique ID:  100942132     Medline TA:  Asian J Androl     Country:  China    
Other Details:
Languages:  eng     Pagination:  566-73     Citation Subset:  IM    
Affiliation:
Department of Histology and Embryology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
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MeSH Terms
Descriptor/Qualifier:
Adult
Bacillaceae / isolation & purification
Bodily Secretions / chemistry,  microbiology
Caulobacteraceae / isolation & purification
Chronic Disease
Humans
Infertility, Male / microbiology*
Least-Squares Analysis
Male
Principal Component Analysis
Prostate / microbiology*,  secretion
Prostatitis / microbiology*
RNA, Bacterial / analysis*
RNA, Ribosomal, 16S / analysis*
Young Adult
Chemical
Reg. No./Substance:
0/RNA, Bacterial; 0/RNA, Ribosomal, 16S
Comments/Corrections

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